Evidence map›Paper›PMID 42149717›Full record

ArticleSTAR protocols2026

Protocol for total RNA sequencing analysis of extracellular RNA from biofluids.

Anneleen Decock, Kimberly Verniers, Philippe Decruyenaere, Annelien Morlion, Jasper Verwilt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Anneleen DecockDepartment of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium.
Kimberly VerniersDepartment of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium.
Philippe DecruyenaereDepartment of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium; Department of Hematology, Ghent University Hospital, 9000 Ghent, Belgium.
Annelien MorlionDepartment of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium. Electronic address: annelien.morlion@ugent.be.
Jasper VerwiltDepartment of Biomedical Sciences, University of Antwerp, 2000 Antwerp, Belgium; Complex Genetics of Alzheimer's Disease Group, VIB Center for Molecular Neurology, 2000 Antwerp, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular or cell-free RNAs (exRNAs) derived from biofluids are utilized in biomarker studies to study health and diseases. Here, we present a protocol for total RNA sequencing analysis of blood plasma exRNA using the Switching Mechanism At 5' end of RNA Template (SMART) cDNA synthesis technology. We describe all steps, from blood plasma preparation to sequencing data analysis. In addition to blood plasma, this protocol can also be applied to exRNA purified from other human, murine, and rat biofluids. For complete details on the use and execution of this protocol, please refer to Decruyenaere et al.

Indexed as

Body FluidsCell-Free Nucleic AcidsRNASequence Analysis, RNAAnimalsHumansMiceRatsCell-Free Nucleic AcidsRNARNA-seqSequence analysisSequencing

Identifiers

PMID42149717
PMCPMC13213309

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.