Evidence map›Paper›PMID 42149716›Full record

ArticleSTAR protocols2026

Protocol for insulin dosing during expansion and maturation of human iPSC-derived cardiomyocytes.

Devin Verbueken, Qianliang Yuan, Vincent A J Warnaar, Shaima Ayubi, Keyin Zhang, Luuk J M Kemna, Luc Bertrand, Steven A J Chamuleau, Jolanda van der Velden, Jan Willem Buikema

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Devin VerbuekenAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands; Department of Cardiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: d.l.verbueken@amsterdamumc.nl.
Qianliang YuanAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands; School of Basic Medical Sciences, Henan University, Kaifeng, China.
Vincent A J WarnaarAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands.
Shaima AyubiAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands.
Keyin ZhangAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands.
Luuk J M KemnaAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands.
Luc BertrandUCLouvain, Intitut de Recherche Expérimentale et Clinique, Pole of Cardiovascular Research, Brussels, Belgium.
Steven A J ChamuleauDepartment of Cardiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
Jolanda van der VeldenAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands.
Jan Willem BuikemaAmsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center, VU University, 1081 HV Amsterdam, the Netherlands; Department of Cardiology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: j.w.buikema@amsterdamumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Techniques for differentiating human induced pluripotent stem cells (hiPSCs) into cardiomyocytes (hiPSC-CMs) can yield up to 98% purity, yet control over hiPSC-CM fate is lacking. Here, we present a protocol for insulin dosing during the expansion and maturation of hiPSC-derived cardiomyocytes. We describe steps for differentiation, purification, replating, cryopreservation, and thawing. We then detail a procedure for expansion using insulin and Wnt/β-catenin signaling, followed by steps to initiate maturation by either removing insulin completely or adding a low dose of insulin. For complete details on the use and execution of this protocol, please refer to Yuan et al.

Indexed as

Cell Culture TechniquesInduced Pluripotent Stem CellsInsulinMyocytes, CardiacCell DifferentiationCryopreservationHumansInsulinCell cultureCell DifferentiationStem Cells

Identifiers

PMID42149716
PMCPMC13213297

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.