ArticleMethods in molecular biology (Clifton, N.J.)2026
Imaging Approach to DNA Damage Induction and Quantification.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Immunofluorescence-based imaging can offer high signal-to-noise and overall superior image quality of fixed cells over that of live-cell imaging. This is especially true of tracking and quantifying DNA damage sites as there is a dearth of reliable or easy-to-use methods in living cells. Here, I describe a useful workflow for DNA damage induction in cell culture, fixation, staining, and method for analyzing DNA double-strand breaks (DSBs). Further, I provide troubleshooting tips for common issues while performing image segmentation and analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.