ArticleMethods in molecular biology (Clifton, N.J.)2026
Purification of Extracellular Vesicles Secreted by Skin Fibroblasts and Keratinocytes.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) secreted by all cell types play crucial roles in intercellular communication through the transfer of bioactive molecules, including proteins, lipids, and nucleic acids, within tissues and throughout the organism. In cutaneous biology, EVs have emerged as key mediators in maintaining skin homeostasis and regulating pathological processes. However, obtaining sufficient quantities of EVs from primary cell cultures remains technically challenging due to inherent limitations in cell number, proliferative capacity, and EV production efficiency compared to immortalized cell lines. In this chapter, we present detailed, step-by-step protocols specifically optimized for isolating small EVs from primary human dermal fibroblasts and epidermal keratinocytes. These methodologies consistently yield sufficient EV quantities for comprehensive functional studies.
Indexed as
Identifiers
42149440What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.