Evidence map›Paper›PMID 42149438›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Validated Step-by-Step Protocols for Human Keratinocyte Isolation and the Construction of Organotypic 3D Epidermal Models.

Jaimy A Klijnhout, Rens H W Peters, Gijs Rikken, Diana Rodijk-Olthuis, Sandrine Dubrac, Yves Poumay, Hanna Niehues, Jos P H Smits, Ellen H van den Bogaard, COST Action CA21108 and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jaimy A KlijnhoutDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.ORCID https://orcid.org/0009-0003-6470-7822
Rens H W PetersDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Gijs RikkenDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Diana Rodijk-OlthuisDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Sandrine DubracDepartment of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria.
Yves PoumayFaculty of Medicine, NAmur Research Institute for LIfe Sciences (NARILIS), University of Namur, Namur, Belgium.
Hanna NiehuesDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Jos P H SmitsDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands.
Ellen H van den BogaardDepartment of Dermatology, Research Institute for Medical Innovation, Radboud University Medical Center (Radboudumc), Nijmegen, The Netherlands. Ellen.vandenBogaard@radboudumc.nl.ORCID https://orcid.org/0000-0003-4846-0287
COST Action CA21108
Next Generation ImmunoDermatology Consortium (NGID)

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional culturing of human keratinocytes at the air-liquid interface yields a fully stratified epidermis, including a functional stratum corneum, and thus enables the study of epidermal structure and function in the context of biomedical, toxicological, and pharmaceutical sciences. Here, we provide an updated step-by-step, detailed protocol for the isolation of human primary keratinocytes and the development of human epidermal equivalents (HEEs) generated from primary keratinocytes or immortalized keratinocytes (N/TERT-1; N/TERT-2G). This chapter includes information on widely accepted procedures for the analysis of epidermal equivalent tissue morphology, keratinocyte proliferation and differentiation, and gene expression. In addition, this chapter handles qualitative and quantitative procedures for measuring epidermal barrier function, including the more recently developed electrical impedance spectroscopy (EIS).

Indexed as

Cell Culture Techniques, Three DimensionalCell SeparationEpidermal CellsEpidermisKeratinocytesCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedHumansTissue EngineeringEpidermisKeratinocyte isolationLaboratory protocolOrganotypic epidermal modelsProliferationSkin barrierSkin tissue engineering

Identifiers

PMID42149438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.