ArticleVeterinary research communications2026
Quercetin modulates Chitinase-3-like protein 1 expression and inflammatory responses in lipopolysaccharide-stimulated buffalo granulosa cells.
Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ovarian inflammatory challenges can impair buffalo fertility by altering granulosa cell (GC) function and oocyte competence. Chitinase-3-like protein 1 (CHI3L1/YKL-40) has been linked to inflammatory processes in several species, and quercetin is a flavonoid with reported anti-inflammatory and antioxidant actions. This study evaluated whether CHI3L1 is responsive to lipopolysaccharide (LPS) stimulation in buffalo granulosa cells and whether quercetin modulates LPS-induced expression of inflammatory markers. Primary buffalo granulosa cells were cultured in vitro and challenged with LPS with or without quercetin co-treatment. CHI3L1 and pro-inflammatory cytokine transcripts (IL-1β, IL-6, and TNF-α) were quantified by real-time PCR; secreted CHI3L1 in culture supernatants was assessed by ELISA; intracellular reactive oxygen species (ROS) were measured by DCFDA fluorescence; and oocyte maturation was assessed using a granulosa cell-cumulus-oocyte complex co-culture system. LPS increased cytokine transcript abundance and ROS production and was accompanied by a modest reduction in oocyte maturation. CHI3L1 mRNA was upregulated following LPS challenge, whereas secreted CHI3L1 protein did not show a significant increase under the conditions tested. Quercetin attenuated LPS-induced cytokine expression and ROS accumulation and partially improved oocyte maturation outcomes. Collectively, these findings identify CHI3L1 as an LPS-responsive transcript in buffalo granulosa cells and support a protective role for quercetin against LPS-induced inflammatory and oxidative responses in vitro. Further work is required to define the functional role of CHI3L1 and the in vivo relevance of quercetin.
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