Evidence map›Paper›PMID 42149092›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2026

Macrophage-Fibroblast Crosstalk Shapes Wound Repair Signaling In Vitro.

Daniela Enriquez-Ochoa, Raji R Nagalla, Yingzi Liu, Christian F Guerrero-Juarez, Hamza Atcha, Gordon Chang, Nancy A Morales, Hannah J Hooper, Arian D Cano, Huy Eng Lim and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Daniela Enriquez-OchoaDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-9985-5716
Raji R NagallaDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-8573-3021
Yingzi LiuDepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-8704-4756
Christian F Guerrero-JuarezDepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-6245-6412
Hamza AtchaDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-7590-8342
Gordon ChangDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Nancy A MoralesDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Hannah J HooperDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Arian D CanoDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Huy Eng LimDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Michael D CahalanDepartment of Physiology and Biophysics, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-4987-2526
Maksim V PlikusDepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-8845-2559
Wendy F LiuDepartment of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.ORCID https://orcid.org/0000-0002-8904-7643

Funding

American Heart Association (AHA) 20PRE35200220California Institute for Regenerative Medicine (CIRM) EDUC4-12822National Heart, Lung, and Blood Institute (NHLBI) 5T32HL116270-03National Institute of Allergy and Infectious Diseases (NIAID) AI142988-01A1National Institute of Allergy and Infectious Diseases (NIAID) R01AI151301National Institute of Allergy and Infectious Diseases (NIAID) R21AI128519-01National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) P30AR075047National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) R21AR077288-01National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) R21AR083769National Institute of General Medical Sciences (NIGMS) T32 GM008620-18NIH Office of the Director (OD) 1S10OD025064-01A1Secretaría de Ciencia, Humanidades, Tecnología e Innovación (Conahcyt) 922586
6 · The paper itself

Abstract

Wound healing requires coordinated interactions between macrophages and fibroblasts, yet how contact-dependent signaling integrates with paracrine pathways to regulate their reciprocal behavior is not well defined. Here, we investigated macrophage-fibroblast communication using complementary 2D and 3D in vitro wound healing models combined with live-cell calcium imaging and single-cell RNA sequencing (scRNA-seq). We show that bone marrow-derived macrophages (BMDMs) promote fibroblast scratch closure in a contact-dependent manner independent of connexins, whereas fibroblasts reciprocally regulate macrophage cytokine secretion through distinct mechanisms. Direct cell-cell contact with fibroblasts enhanced macrophage IL10 production via connexin 43 (Cx43)-dependent signaling, whereas TNFα secretion was suppressed through paracrine interactions. We further demonstrate that fibroblast-macrophage contact induces connexin-dependent intermittent calcium signals selectively in macrophages. ScRNA-seq revealed that wounding reshapes macrophage and fibroblast populations, uncovering dynamic regulation of cell adhesion molecules (CAMs) and intercellular signaling pathways. Together, these findings reveal the integration of contact-dependent calcium and connexin signaling with transcriptional remodeling to coordinate macrophage-fibroblast behavior during healing.

Indexed as

Cell CommunicationFibroblastsMacrophagesSignal TransductionWound HealingAnimalsCalciumCalcium SignalingConnexin 43MiceMice, Inbred C57BLCalciumConnexin 43calcium signalingcell–cell contactconnexin 43fibroblastsmacrophagessingle‐cell RNA sequencingwound healing

Identifiers

PMID42149092
PMCPMC13182763

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.