A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease.

Jiuxing Li, Rudi Liu, Wenqing Zhang, Bruno J Salena, Yingfu Li

Abstract read

In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Jiuxing LiCentral Hospital of Dalian University of Technology, Dalian, Liaoning, China.ORCID 0000-0001-7012-2482

Rudi LiuCentral Hospital of Dalian University of Technology, Dalian, Liaoning, China.

Wenqing ZhangDepartment of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, School of Biomedical Engineering, Biointerfaces Institute, McMaster University, Hamilton, Ontario, Canada.

Bruno J SalenaDepartment of Medicine, McMaster University, Hamilton, Ontario, Canada.

Yingfu LiDepartment of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, School of Biomedical Engineering, Biointerfaces Institute, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0002-7533-6743

Funding

Doctoral Scientific Research Foundation of Liaoning Province 2025-BS-0022Doctoral Scientific Research Foundation of Liaoning Province 2025-BS-0989Fundamental Research Funds for the Central Universities DUT25RC(3)033Natural Sciences and Engineering Research Council of Canada RGPIN-2020-06401

6 · The paper itself

Abstract

Rapid bacterial detection, including multiplex formats, is critical for clinical diagnostics, particularly when distinct pathogens cause similar symptoms and complicate differential diagnosis. Here we report a portable gold-coated filter tip-based assay (GFTA) for rapid, colorimetric, and two-plex bacterial detection. Cleavable functional nucleic acid (FNA) probes were obtained through in vitro selection to target RNase H2, a conserved bacterial endoribonuclease. In GFTA, horseradish peroxidase (HRP) serves as the reporter, while a bacterium-specific FNA probe functions both as the recognition element and as a molecular bridge linking HRP to a gold-coated filter tip. When a target bacterium is present, its RNase H2 cleaves the FNA probe and releases HRP. The released enzyme is then captured by a second gold-coated filter tip, where it catalyzes tetramethylbenzidine oxidation to generate a visible color change. The assay is completed within 30 min. GFTA enabled specific detection of Clostridioides difficile and Salmonella typhimurium at concentrations as low as as low as 1.3 × 10

Indexed as

Clostridioides difficileColorimetryEndoribonucleasesNucleic Acid ProbesRibonuclease HSalmonella typhimuriumBiosensing TechniquesGoldHorseradish PeroxidaseHumansRapid Diagnostic TestsEndoribonucleasesGoldHorseradish PeroxidaseNucleic Acid ProbesRibonuclease Hbacterial detectionbiosensorfunctional nucleic acidgold‐coated filter tip‐based assay (GFTA)RNase H2

Identifiers

PMID42148598

DOI10.1002/anie.5320160

PMCPMC13340475

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.