Evidence map›Paper›PMID 42148575›Full record

ArticleMicrobiology spectrum2026

A CRISPR interference system for tunable gene expression integrated with a promoter library for

Byeongchan Kang, Ji-Yeon Kim, Soyoung Oh, Jina Kweon, Hongseo Park, Minji Kim, In-Geol Choi, In Seop Chang

Abstract read
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In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Byeongchan Kang *Department of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.ORCID 0009-0003-0359-4756
Ji-Yeon Kim *Department of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.ORCID 0000-0002-1425-1754
Soyoung OhDepartment of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.
Jina KweonDepartment of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.
Hongseo ParkDepartment of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.
Minji KimDepartment of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.
In-Geol ChoiDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seongbuk-gu, Seoul, Republic of Korea.ORCID 0000-0001-7403-6274
In Seop ChangDepartment of Environment and Energy Engineering, Gwangju Institute of Science and Technology, Buk-gu, Gwangju, Republic of Korea.ORCID 0000-0001-5064-7951

Funding

National Research Foundation of Korea RS-2021-NR060081National Research Foundation of Korea RS-2023-00249146
6 · The paper itself

Abstract

Acetogens are key biocatalysts for carbon-neutral biorefineries, yet their metabolic engineering is limited by the lack of tunable transcriptional regulation systems. Here, we developed a synthetic promoter library for IMPORTANCE: Transitioning to a carbon-neutral economy requires biocatalysts that can efficiently convert waste-derived substrates into valuable products. Acetogens are industrially relevant organisms for gas fermentation, but the lack of genetic toolkits tailored to their physiology has constrained metabolic engineering. We present the first synthetic promoter-CRISPRi platform specifically optimized for

Indexed as

CRISPR-Cas SystemsPromoter Regions, GeneticBacterial ProteinsClustered Regularly Interspaced Short Palindromic RepeatsGene Expression Regulation, BacterialGene LibraryMetabolic EngineeringBacterial ProteinsCRISPR interferencedead-Cas9Eubacterium callanderi KIST612promoter librarysynthetic promoter

Identifiers

PMID42148575
PMCPMC13340025

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.