ArticleHealth science reports2026
Molecular Characterization of
Article in Health science reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Background and Aims: Tuberculosis (TB) remains a major global health problem and poses significant challenges for diagnosis and treatment. The World Health Organization (WHO) recommends the use of molecular methods for the diagnosis and genotyping of Methods: In total, 643 suspected cases of TB were collected from March 2022 to November 2023. Acid-fast bacilli (AFB) were detected using Ziehl-Neelsen (ZN) staining, fluorochrome staining, and culture on Löwenstein-Jensen medium. GeneXpert MTB/RIF method was applied to detect MTB and RR-TB. Finally, 15-locus-based MIRU-VNTR was used for molecular genotyping. Results: Out of 643 specimens, 20/643 (3.11%) specimens were diagnosed as positive for MTB by culture. GeneXpert MTB/RIF confirmed RR-TB in one sample. Positive isolates were placed in nine different clusters using MIRU-VNTR genotyping. All isolates were not assignable to the sublineages in the MIRU-VNTRplus database but were close to the Delhi/CAS. Conclusion: The high clustering rate in our results indicates that TB transmission in this region originates from multiple sources and reflects active transmission rather than reactivation of latent TB. In addition, the lineages of MTB isolates identified in Ilam were closely related to those reported from other Iranian provinces and neighboring countries, indicating epidemiological links. Improving diagnostic capacity and implementing effective control measures are essential to reduce TB transmission.
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