Evidence map›Paper›PMID 42147454›Full record

ArticleTransboundary and emerging diseases2026

Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain.

Yuhong Yang, Ruichen Wang, Weijia Zhang, Qikai Yin, Fan Li, Shihong Fu, Kai Nie, Qianqian Cui, Songtao Xu, Huanyu Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuhong YangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.ORCID https://orcid.org/0009-0003-9979-2166
Ruichen WangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Weijia ZhangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Qikai YinNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Fan LiNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Shihong FuNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Kai NieNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Qianqian CuiNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Songtao XuNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.
Huanyu WangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, Beijing Key Laboratory of Viral Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, chinacdc.cn.ORCID https://orcid.org/0000-0001-6245-2027

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The recent emergence of the genotype five Japanese encephalitis virus (G5 JEV) has once again drawn public attention to public health concerns. Plaque assay analysis of G5 JEV parental strain XZ0934 revealed two distinct plaque morphologies. To investigate this phenotypic heterogeneity, we performed single-plaque purification and isolated two strains, designated as XZ0934-L (large plaque) and XZ0934-S (small plaque). Subsequently, deep mutational scanning revealed an amino acid mutation at position 138 (E138K) in the E protein of the XZ0934-S strain. Viral titer determination and plaque morphology analysis showed that the titers of XZ0934-L and XZ0934-S in BHK-21 cells were 10

Indexed as

Encephalitis, JapaneseEncephalitis Virus, JapaneseTranscriptomeAnimalsCell LineCricetinaeGenotypeMutationE138K mutationgenotype 5 Japanese encephalitis virustranscriptomic characterization

Identifiers

PMID42147454
PMCPMC13173371

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.