ArticleResearch square2026
Plasma Heparan Sulfate Structural Variation and Phenotypic Heterogeneity in Pediatric Acute Respiratory Distress Syndrome.
Colin J Sallee, Aline B Maddux, Joseph A Hippensteel, Daniela Markovic, Zhangjie Wang, Jian Liu, Kaori Oshima, Robert P Richter, Andreas Schwingshackl, Peter M Mourani and 3 more
Abstract readPreprint
In one paragraphArticle in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
13 authors.
Colin J SalleeUniversity of California Los Angeles and Mattel Children's Hospital.
Aline B MadduxUniversity of Colorado School of Medicine and Children's Hospital Colorado.
Joseph A HippensteelUniversity of Colorado Anschutz Medical Campus.
Daniela MarkovicUniversity of California Los Angeles.
Zhangjie WangGlycan Therapeutics.
Jian LiuUniversity of North Carolina at Chapel Hill.
Kaori OshimaHarvard Medical School and Massachusetts General Hospital.
Robert P RichterUniversity of Alabama at Birmingham Heersink School of Medicine.
Andreas SchwingshacklUniversity of California Los Angeles and Mattel Children's Hospital.
Peter M MouraniUniversity of Michigan Medical School.
David ElashoffUniversity of California Los Angeles.
Eric P SchmidtHarvard Medical School and Massachusetts General Hospital.
Anil SapruUniversity of California Los Angeles and Mattel Children's Hospital.
Funding
Pediatric Critical Care Scientist Development ProgramK12HD047349 · NICHD · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI HEATHER T. KEENAN, Kevin M Watt · 2004 to 2026
$17.0MEndothelial glycocalyx reconstitution during sepsisR01HL125371 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI LINHARDT, ROBERT J, SCHMIDT, ERIC PETER · 2014 to 2018
$3.4MAncillary to ABC PICU to study MOD in critically ill childrenR01HD092471 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PINEDA SOTO, JOSE ALFREDO, SAPRU, ANIL · 2017 to 2021
$2.4MRechargeable Anti-thrombogenic Films for Blood Contacting ApplicationsR01HL171101 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Elliot Chaikof · 2024 to 2026
$2.2MDevelopment of analytical methods for glycosaminoglycans from biological sourcesR44GM142304 · NIGMS · GLYCAN THERAPEUTICS CORPORATION · PI WANG, ZHANGJIE · 2022 to 2023
$2.0MTargeting circulating endothelial glycocalyx fragments to reduce septic encephalopathyR01GM125095 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI HERSON, PACO S, SCHMIDT, ERIC PETER · 2017 to 2020
$1.9MMechanisms driving endothelial angiopoietin-2 expression and vascular dysfunction during pediatric sepsisK08GM144788 · NIGMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI RICHTER, ROBERT P · 2021 to 2025
$930kLung-Brain Axis as a Mediator of DeliriumK08HL159353 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI HIPPENSTEEL, JOSEPH ALAN · 2021 to 2025
$809kFunctional Outcomes after Prolonged Mechanical Ventilation in ChildrenK23HD096018 · NICHD · UNIVERSITY OF COLORADO DENVER · PI MADDUX, ALINE BERNARD · 2019 to 2023
$807kNHLBI NIH HHS K08 HL159353NHLBI NIH HHS R01 HL125371NHLBI NIH HHS R01 HL171101NICHD NIH HHS K12 HD047349NICHD NIH HHS K23 HD096018NICHD NIH HHS R01 HD092471NIGMS NIH HHS K08 GM144788NIGMS NIH HHS R01 GM125095NIGMS NIH HHS R44 GM142304
6 · The paper itselfAbstract
Endothelial glycocalyx (eGCX) shedding contributes to microvascular endotheliopathy in Acute Respiratory Distress Syndrome (ARDS) and is a potential underrecognized source of phenotypic heterogeneity. In pediatric ARDS (PARDS), we examined whether circulating heparan sulfate (HS) signatures, as readouts of eGCX shedding, capture inter-individual variability beyond other eGCX components and protein biomarkers, whether specific HS structural features are enriched, and whether they correlate with heparanase-1 (HPSE) activity. We retrospectively analyzed plasma samples (2018-2020) from children with and without PARDS. Mass spectrometry quantified glycosaminoglycans and sulfation subtypes alongside HPSE activity, while protein biomarkers were measured by multiplex assay. Among 46 children (36 PARDS, 10 no PARDS), principal component analysis identified three components explaining 63% of variance. The primary component (PC1) was driven by 6-
Indexed as
Biological HeterogeneityEndothelial GlycocalyxHeparanaseHeparan SulfateMass SpectrometryPediatric Acute Respiratory Distress Syndrome
Identifiers
PMID42147147
PMCPMC13174808
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390