ArticlebioRxiv : the preprint server for biology2026
Molecular Structure and DNA Binding Mode of Unsymmetric Cyanine Dyes RiboGreen and OliGreen.
Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The binding of fluorescent dyes to nucleic acids and their fluorogenic properties are indispensable tools for nucleic acid detection, quantification, and imaging, yet the molecular structures of several widely used commercial dyes have remained unknown. Here, we Highlights: Determination of the molecular structures of nucleic acid dyes RiboGreen, OliGreen, and PicoGreenNMR spectroscopic characterization of all three dyes.NMR and MD data indicate binding to be dominated by electrostatic and π-π stacking interactions.
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