Evidence map›Paper›PMID 42146559›Full record

ArticlebioRxiv : the preprint server for biology2026

Genome-wide CRISPR knockout cell screening platform for the disease vector tick species

Matthew Butnaru, William McKenna, Srishti Goswami, Alejandra Wu-Chuang, Enzo Mameli, Abigail Wilcox, Léopoldine Quennesson, Ah-Ram Kim, Austin Veal, Weihang Chen and 10 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Matthew ButnaruDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
William McKennaDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
Srishti GoswamiDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0009-0003-4442-4577
Alejandra Wu-ChuangDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.ORCID 0000-0002-6020-2128
Enzo MameliDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-0172-8090
Abigail WilcoxDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0009-0000-0856-7122
Léopoldine QuennessonDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
Ah-Ram KimDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0001-9597-6759
Austin VealDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
Weihang ChenDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
Hugo VerzoneDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0009-0005-5249-748X
Elizabeth A LaneDepartment of Genetics, Harvard Medical School, Boston, MA 02115.
Hanna J Laukaitis-YouseyDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.ORCID 0000-0002-2267-4634
Chad AraneoDepartment of Immunology, Harvard Medical School, Boston, MA 02115.
Nisha SinghDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.
Joao H F PedraDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201.ORCID 0000-0001-8740-2667
Yanhui HuDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0003-1494-1402
Raghuvir ViswanathaDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-9457-6953
Norbert PerrimonDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0001-7542-472X
Stephanie E MohrDepartment of Genetics, Harvard Medical School, Boston, MA 02115.ORCID 0000-0001-9639-7708

Funding

Tick Resources CoreP01AI138949 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI Erol Fikrig, UTPAL PAL · 2018 to 2026
$13.5M
Ubiquitylation and Rickettsial Colonization of a Tick VectorR01AI116523 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI PEDRA, JOAO · 2016 to 2025
$6.2M
Characterization of innate immune signaling pathways in the tick Ixodes scapularisR01AI186279 · NIAID · HARVARD MEDICAL SCHOOL · PI STEPHANIE E MOHR, NORBERT PERRIMON · 2025 to 2026
$1.6M
Establishing CRISPR cell screening for the deer tick Ixodes scapularis, a vector of Lyme and other diseasesR21AI168592 · NIAID · HARVARD MEDICAL SCHOOL · PI MOHR, STEPHANIE E, PEDRA, JOAO · 2022 to 2023
$469k
NIAID NIH HHS P01 AI138949NIAID NIH HHS R01 AI116523NIAID NIH HHS R01 AI186279NIAID NIH HHS R21 AI168592
6 · The paper itself

Abstract

The black legged tick,

Indexed as

black-legged tickcell line engineeringCRISPR-Cas screeningfunctional genomicsISE18ISE6Ixodes scapularisnon-model organisms

Identifiers

PMID42146559
PMCPMC13174539

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.