Evidence map›Paper›PMID 42146352›Full record

ArticlebioRxiv : the preprint server for biology2026

dCas9-metabolic enzyme fusions modulate global and locus-specific gene expression.

Kellen V Biesbrock, Spencer A Haws, Harshini Cormaty, Rupa Sridharan, John M Denu

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kellen V BiesbrockWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715, USA.
Spencer A HawsWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715, USA.
Harshini CormatyWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715, USA.
Rupa SridharanWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715, USA.
John M DenuWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715, USA.ORCID 0000-0001-9415-0365

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Justine Yang Bruce · 1985 to 2026
$142.6M
Dynamics and molecular mechanisms linking metabolism and the epigenomeR35GM149279 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI JOHN M DENU · 2023 to 2026
$2.8M
Dietary regulation of the hepatic epigenomeR01DK125859 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI DENU, JOHN M, LAMMING, DUDLEY WILLIAM · 2021 to 2023
$1.8M
NCI NIH HHS P30 CA014520NIDDK NIH HHS R01 DK125859NIGMS NIH HHS R35 GM149279
6 · The paper itself

Abstract

Central metabolites function as essential co-substrates for chromatin-modifying enzymes, directly linking cellular metabolism to chromatin regulation. Accordingly, whole-cell fluctuations in co-substrate availabilities have been shown to promote diverse phenotypes through chromatin-dependent mechanisms. There is emerging evidence that metabolic enzymes producing co-substrates for chromatin modifying enzymes can exist in the nucleus, suggesting that nucleus-specific metabolite availability regulates chromatin state. Here, we developed CRISPRm (CRISPR metabolite) to assess how nucleus-specific metabolic perturbations influence chromatin function. Five dCas9-metabolic enzyme fusions (

Indexed as

acetylationCRISPRdCas9epigeneticshistonemetabolismmethylationtranscription

Identifiers

PMID42146352
PMCPMC13174488

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.