Evidence map›Paper›PMID 42146340›Full record

ArticlebioRxiv : the preprint server for biology2026

Improving positively tuned voltage indicators for faster kinetics and higher contrast.

Sungmoo Lee, Guofeng Zhang, Yukun Alex Hao, Richard H Roth, Yue Sun, Can Dong, Jun Zhu, Laura C Gomez, Ryan G Natan, Dongyun Jiang and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Sungmoo LeeDepartment of Neurobiology, Stanford University, Stanford, CA, USA.ORCID 0000-0001-5518-6488
Guofeng ZhangDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Yukun Alex HaoDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Richard H RothDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.ORCID 0000-0002-6855-999X
Yue SunDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Can DongDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Jun ZhuDepartment of Neuroscience, University of California, Berkeley, CA, USA.ORCID 0000-0002-7905-7856
Laura C GomezDepartment of Neuroscience, University of California, Berkeley, CA, USA.
Ryan G NatanDepartment of Neuroscience, University of California, Berkeley, CA, USA.
Dongyun JiangDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Lan Xiang LiuDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Guilherme Testa-SilvaInstitute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Atsuki HiramotoInstitute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Botond RoskaInstitute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Daniel FeldmanDepartment of Neuroscience, University of California, Berkeley, CA, USA.ORCID 0000-0003-4646-8170
Na JiDepartment of Neuroscience, University of California, Berkeley, CA, USA.
Thomas R ClandininDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Lisa GiocomoDepartment of Neurobiology, Stanford University, Stanford, CA, USA.
Jun DingDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Michael Z LinDepartment of Neurobiology, Stanford University, Stanford, CA, USA.ORCID 0000-0002-0492-1961

Funding

Resource Core 3 - Teaching/Training/Dissemination Core (TTDC)U19NS118284 · NINDS · STANFORD UNIVERSITY · PI GIOCOMO, LISA · 2021 to 2025
$18.1M
Reexamining the Role of Dendrites in Neuronal FunctionRM1NS132981 · NINDS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Jayeeta Basu, RAFAEL YUSTE · 2023 to 2026
$10.3M
Optimization of genetically encoded voltage and neurotransmitter indicators for multiwavelength in vivo analysis of brain circuitsUM1MH136462 · NIMH · MAX PLANCK FLORIDA CORPORATION · PI Michael Z. Lin, Kaspar Podgorski · 2024 to 2026
$6.3M
The power of positivity: a novel class of voltage indicators for high-fidelity brain activity imagingR01NS123681 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2021 to 2021
$3.6M
Optimizing FACED for robust large-scale activity recordingU01NS137449 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI NA Ji · 2024 to 2026
$2.1M
The Dynamics of Neural Representations for Distinct Spatial Contexts and Memory EpisodesR01MH126904 · NIMH · STANFORD UNIVERSITY · PI Lisa Giocomo · 2022 to 2026
$2.0M
Precise neuromodulation for encoding reward in the hippocampusR01MH130452 · NIMH · STANFORD UNIVERSITY · PI Lisa Giocomo · 2022 to 2026
$2.0M
Adaptive optical microscopy for high-accuracy recording of neural activity in vivoU01NS118300 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA · 2021 to 2023
$1.7M
Identifying new sensors for in vivo cochlear imagingR21DC020325 · NIDCD · STANFORD UNIVERSITY · PI RICCI, ANTHONY J · 2022 to 2023
$437k
NIDCD NIH HHS R21 DC020325NIMH NIH HHS R01 MH126904NIMH NIH HHS R01 MH130452NIMH NIH HHS UM1 MH136462NINDS NIH HHS R01 NS123681NINDS NIH HHS RM1 NS132981NINDS NIH HHS U01 NS118300NINDS NIH HHS U01 NS137449NINDS NIH HHS U19 NS118284
6 · The paper itself

Abstract

Existing positively tuned ASAP-family voltage indicators such as ASAP4e exhibit superior photostability compared to the negatively tuned ASAP3 and ASAP5, but signal-to-noise ratios for spike detection remained similar to ASAP3. To improve spike detection by positively tuned ASAP indicators, we performed multiple rounds of structure-guided saturation mutagenesis of an ASAP4e predecessor while screening directly for faster responses. One variant, ASAP6c, demonstrated ~100% increases in fluorescence in response to single action potentials in both one-photon and two-photon imaging in vivo, achieving a 3-fold improvement in signal-to-noise ratios over ASAP4e.

Identifiers

PMID42146340
PMCPMC13174376

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.