Evidence map›Paper›PMID 42146049›Full record

ArticleFrontiers in veterinary science2026

Engineering a 3D matrix from porcine plasma for enhanced cell proliferation and differentiation.

Yangyang Ma, Xiaohui Peng, Yuting Lin, Tiantian Xia, Qianqian Pan, Yuhang Wu, Mingjing Cui, Shuaifeng Shen, Fugui Fang, Yunsheng Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yangyang Ma *College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Xiaohui Peng *College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yuting LinCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Tiantian XiaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Qianqian PanCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yuhang WuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Mingjing CuiCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Shuaifeng ShenCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Fugui FangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yunsheng LiCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Zubing CaoCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yunhai ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Tong YuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The limited availability of suitable scaffolds for three-dimensional (3D) cell culture has driven the development of new biomaterials. In this study, we developed a porcine fibrin matrix gel (PFMG) derived entirely from porcine blood as a species-matched 3D scaffold that integrates the structural support of fibrin with the bioactive components of porcine platelet lysate (PPL). Methods: PFMG was prepared from porcine plasma supplemented with PPL and calcium to induce gelation. Its physicochemical properties, including tunable stiffness, fibrillar microstructure, and molecular sieve-like behavior, were characterized by coagulation testing, diffusion assays, scanning electron microscopy, and rheological analysis. The biological performance of PFMG was evaluated using porcine mesenchymal stem cells (MSCs) and porcine embryonic fibroblasts (PEFs), and compared with conventional two-dimensional (2D) cultures supplemented with fetal bovine serum (FBS) or PPL. Cell proliferation, MSC tri-lineage differentiation, and transcriptomic changes were assessed. Results: PFMG formed a stable fibrin-based 3D network with adjustable mechanical properties and selective permeability to small molecules. Compared with conventional 2D culture systems, PFMG significantly enhanced the proliferation of both MSCs and PEFs and supported a characteristic 3D cellular morphology. MSCs cultured in PFMG retained adipogenic, osteogenic, and chondrogenic differentiation potential, and adipogenic differentiation was further enhanced when induction was performed with PPL instead of FBS. Transcriptomic analysis of MSCs cultured in PFMG revealed a distinct gene-expression profile characterized by upregulation of genes involved in cell-cycle progression, oxidative phosphorylation, and growth-related pathways, alongside downregulation of genes associated with cell adhesion and extracellular matrix interactions. Discussion: These findings indicate that PFMG provides a supportive, species-specific, and cost-effective 3D microenvironment for porcine cell culture. By combining scaffold function with platelet-derived bioactivity, PFMG promotes cell expansion while preserving multilineage differentiation capacity. This fully porcine platform therefore represents a promising biomaterial for porcine cell culture, tissue engineering, and regenerative medicine applications.

Indexed as

3D cell culturecell proliferationmesenchymal stem cellsplatelet lysateporcine fibrin matrix gel

Identifiers

PMID42146049
PMCPMC13171798

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.