Evidence map›Paper›PMID 42145849›Full record

ArticleAnemia2026

Microtubular Disruption Alters Intestinal Permeability in Murine Neonatal Anemic Intestine.

Marie Amalie Balamurugan, Balamurugan Ramatchandirin, Juanitaa George Raj, Arjun Subrramanya, Sayma Azeem, Zainab D Lawal, Megan M Ferris, Elizabeth Meister, Krishnan MohanKumar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Anemia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marie Amalie BalamuruganDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.
Balamurugan RamatchandirinDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.
Juanitaa George RajDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.
Arjun SubrramanyaDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.
Sayma AzeemDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.
Zainab D LawalDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA, unmc.edu.
Megan M FerrisDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA, unmc.edu.
Elizabeth MeisterDepartment of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA, unmc.edu.
Krishnan MohanKumarDepartment of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, 75390, USA, utsouthwestern.edu.ORCID https://orcid.org/0000-0003-1036-5188

Funding

Liver-Gut Axis in Neonatal Anemia and Its Role in RBC Transfusion Associated Gut InjuryR01HL163043 · NHLBI · UT SOUTHWESTERN MEDICAL CENTER · PI Mohan Kumar Krishnan · 2023 to 2026
$1.9M
RBC transfusion exacerbates brain inflammation in anemic murine neonates and causes long term neurodevelopment impairmentR01HD116950 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI Mohan Kumar Krishnan · 2025 to 2026
$876k
RBC TRANSFUSION IN ANEMIC NEONATES LEADS TO SYSTEMIC INFLAMMATORY RESPONSE SYNDROMER21HD105880 · NICHD · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI KRISHNAN, MOHAN KUMAR · 2021 to 2022
$433k
NHLBI NIH HHS R01 HL163043NICHD NIH HHS R01 HD116950NICHD NIH HHS R21 HD105880
6 · The paper itself

Abstract

Anemia is a frequent diagnosis in premature and critically ill infants and is caused by phlebotomy, which is essential for medical care. The severity of anemia often correlates with the acuity of illness. We recently showed that severe neonatal anemia causes a "leaky gut" in neonatal mouse pups by altering the structure and function of epithelial adherens junctions through decreased E-cadherin expression. We hypothesize that microtubules play a major role in maintaining adherens junction integrity. To test this hypothesis, C57BL/6 mice were subjected to timed phlebotomy between postnatal days (P) 2-10 to induce severe anemia (hematocrits 20%-24%). Microtubular assembly was evaluated by electron microscopy, quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry, and/or immunoblotting on intestinal tissues, Caco-2 intestinal epithelial-like cells, and colonic organoids. The electron micrographs showed loss of microtubules in the anemic intestine and disrupted intestinal epithelial microtubular assembly. Microtubular disruption was associated with decreased expression of the α-tubulin 4a (tuba4a) subunit. The observed ultrastructural changes that disrupt epithelial barrier functions result from hypoxia-induced microRNA let-7e destabilizing tuba4a expression in the anemic intestinal epithelium. In conclusion, phlebotomy-induced anemia in mouse neonates is associated with a "leaky gut" by the disruption of microtubular assembly.

Indexed as

microtubular assemblyneonatal micephlebotomy-induced anemiatubulin

Identifiers

PMID42145849
PMCPMC13175075

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.