Evidence map›Paper›PMID 42144636›Full record

ArticleJournal of animal science and biotechnology2026

Depicting the dynamic transcriptional and epigenetic landscape of testis development in pubertal Simmental cattle.

Junmei Zhang, Xin Qi, Wenxuan Zhao, Shijie Yuan, Na Hai, Leqian Yu, Jinlian Hua, Dawei Yu, Yulei Wei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junmei Zhang *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Xin Qi *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Wenxuan ZhaoState Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China.
Shijie YuanState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Na HaiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Leqian YuState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
Jinlian HuaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Dawei YuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100193, China. ydw023@163.com.
Yulei WeiState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. yulei.wei@cau.edu.cn.

Funding

STI 2030-Major Projects 2023ZD0407504
6 · The paper itself

Abstract

backgroundThe reproductive development of Simmental cattle, a vital breed for global beef production, remains poorly understood at the molecular and cellular levels. A systematic analysis of the regulatory mechanisms governing germ cell fate transitions during testicular development is essential for advancing breeding efficiency and reproductive technologies in cattle.

resultsUsing integrated single-cell RNA sequencing (scRNA-seq) and single-nucleus ATAC sequencing (sNucATAC-seq) on testicular tissues from Simmental cattle across postnatal (PN), prepubertal (PP), and pubertal (PUB) stages, we identified core transcriptional regulators-including E2F1, BCLAF1, and YY1-that govern germ cell fate transitions. Several signaling pathways, such as TGF-β, MAPK, ErbB, and AMPK, were found to participate in spermatogenic processes. Sertoli cells were classified into three functional subtypes: Stage 1 is associated with the transition from spermatogonial stem cells (SSCs) to differentiating spermatogonia (Diff-SPG), Stage 2 correlates with development from Diff-SPG to spermatocytes (SPC), and Stage 3 coincides with the transformation from SPC to spermatids (SPT). Cross-species comparative transcriptomics with humans, pigs, and mice revealed conserved pathways in germ cell development, with E2F1 notably conserved during the SSC-to-Diff-SPG transition.

conclusionsThis study deciphers the regulatory network controlling germ cell fate transitions during bovine testicular development. The identification of conserved regulators and pathways provides novel insights into spermatogenesis and supports the development of strategies to overcome meiotic barriers in stem cell systems. These findings pave the way for precision breeding and advanced reproductive technologies in mammals.

Indexed as

Simmental cattleSingle-cell RNA sequencingSingle-nucleus ATAC sequencingSpermatogenesis

Identifiers

PMID42144636
PMCPMC13181952

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.