ArticleVeterinary clinical pathology2026
Loss of Stability for Blood Urea Nitrogen in Cattle Serum Samples Stored for 3 and 6 Months at -20°C and -80°C.
Article in Veterinary clinical pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundBiological samples are stored for delayed or add-on analyses. However, storage conditions, including time and temperature, can affect the stability of measurands in the samples.
objectivesTo assess the stability of blood urea nitrogen (BUN) in cattle serum samples.
methodsBlood samples collected from 72 heifers were immediately analyzed for BUN by a commercial laboratory and our in-house laboratory (optimal conditions, t
resultsOur in-house analytical methodology performed within the ASVCP recommended guidelines. BUN (mmol/L) ranged from 1.9 to 5.4 in the commercial laboratory and 1.6 to 6.1 in our in-house laboratory. There was poor agreement with proportional bias in which the higher the BUN concentration, the greater the difference between laboratories. Storage at -20°C and -80°C yielded PD% between -18.6% and 27.1% after 3 months and -17.5% and 71.6% after 6 months. Regardless of freezing temperature, 54% and 80% of PD% values were greater than the MPI after 3- and 6-month's storage, respectively.
conclusionsAlthough cattle serum sample degradation begins by three months storage time, further studies are needed to establish the actual stability limit for BUN.
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