Evidence map›Paper›PMID 42143231›Full record

ArticleBMC genomics2026

Molecular characterization of two conjugative resistance plasmids from a clinical multidrug-resistant ST15 Klebsiella pneumoniae.

Ruixue Tao, Meng Li, Nan Zhang, Linke Cao, Jie Zhang, Hanqi Li, Wenbo Yan, Yiming Yang, Tietao Wang, Yani Zhang and 1 more

Abstract read
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In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Ruixue Tao *Affiliated Chang'an Hospital of Northwest University, Xi'an, Shaanxi, 710100, People's Republic of China.
Meng Li *Science and Education Department, Xi'an Fifth Hospital, Xi'an, Shaanxi, People's Republic of China. limengde2021@163.com.
Nan ZhangKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China.
Linke CaoKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China.
Jie ZhangScience and Education Department, Xi'an Fifth Hospital, Xi'an, Shaanxi, People's Republic of China.
Hanqi LiPopulation Health Sciences, Institute of Epidemiology and Health Care, University College London, Gower Street, London, UK.
Wenbo YanKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China.
Yiming YangKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China.
Tietao WangKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China.
Yani ZhangKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Shaanxi, Xi'an, People's Republic of China. yani@nwu.edu.cn.
Ke XingAffiliated Chang'an Hospital of Northwest University, Xi'an, Shaanxi, 710100, People's Republic of China. 514867570@qq.com.

Funding

Natural Science Basic Research Program of Shaanxi 2025JC-QYCX-029Xi'an Fifth Hospital 2024dx02
6 · The paper itself

Abstract

The increasing emergence of multidrug-resistant (MDR) Klebsiella pneumoniae strains has become a threat to public health, and it is important to understand the mechanisms underlying adaptive evolution. In this study, we characterized a clinical MDR isolate of K. pneumoniae, designated KPA1, which belongs to sequence type 15 (ST15), along with its two associated resistance plasmids, pKPA1-1 and pKPA1-2. Conjugation assays demonstrated that both plasmids were capable of self-transfer. Notably, pKPA1-1 could be transferred to both K. pneumoniae and Escherichia coli, while pKPA1-2 exhibited a restricted transfer capability, being transferable only to K. pneumoniae. Antimicrobial susceptibility testing, along with plasmid stability assays, indicated that the resistance spectrum of pKPA1-2 similar to that of pKPA1-1, and pKPA1-2 was more stable than pKPA1-1. In addition, virulence assays revealed that the acquisition of either plasmid resulted in a reduction in bacterial pathogenicity. This study elucidates the molecular characterization of conjugative resistance plasmids pKPA1-1 and pKPA1-2. These findings shed light on the adaptive mechanisms underlying multidrug resistance in K. pneumoniae.

Indexed as

Conjugation, GeneticDrug Resistance, Multiple, BacterialKlebsiella pneumoniaePlasmidsAnti-Bacterial AgentsEscherichia coliHumansKlebsiella InfectionsMicrobial Sensitivity TestsAnti-Bacterial AgentsConjugative plasmidKlebsiella pneumoniaeMobile genetic elementMultidrug-resistanceST15

Identifiers

PMID42143231
PMCPMC13348810

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.