Evidence map›Paper›PMID 42143145›Full record

ArticleScientific reports2026

Evaluation of methods for early detection of Tropilaelaps mites in European honey bee (Apis mellifera) colonies.

Cooper Nat Schouten, Kelly Lees, John M K Roberts, Emma Thompson Brewster, Flemin Gabriel, Koi Tonny

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cooper Nat SchoutenFaculty of Science and Engineering, Southern Cross University, Military Road, East Lismore, NSW, Po 1572480, Australia. cooper.schouten@scu.edu.au.ORCID http://orcid.org/0000-0003-4398-9499
Kelly LeesDepartment of Primary Industries and Regional Development, Tocal, NSW, Australia.
John M K RobertsAustralian Capital Territory, Commonwealth Scientific and Industrial Research Organisation, Canberra, 2601, Australia.
Emma Thompson BrewsterFaculty of Science and Engineering, Southern Cross University, Military Road, East Lismore, NSW, Po 1572480, Australia.
Flemin GabrielCoffee Industry Cooperation Limited, Goroka, Eastern Highlands Province, Papua New Guinea.
Koi TonnyCoffee Industry Cooperation Limited, Goroka, Eastern Highlands Province, Papua New Guinea.

Funding

Australian Centre for International Agricultural Research LS/2014/042
6 · The paper itself

Abstract

Tropilaelaps mites are emerging ectoparasitic parasites of European honey bees (Apis mellifera) that pose a serious threat to apiculture and pollination security globally. There is a paucity of evidence to support current detection methods used in biosecurity programs. We conducted field trials in Papua New Guinea using 27 infested A. mellifera colonies across six apiaries to evaluate six Tropilaelaps detection methods: bump test, alcohol wash, modified rapid brood uncapping, worker brood uncapping, environmental DNA (eDNA) swabs, and sticky mats with an acaricide. eDNA swabs and acaricides with sticky mats had the highest sensitivity detecting Tropilaelaps in 100% and 92% of the tested infested colonies respectively, while the alcohol wash and bump test had a sensitivity of 41% and 33% in the tested hives. Cost analysis identified rapid brood uncapping as the most cost-effective method (A$6.87 per test), while eDNA swabs and acaricide-treated sticky mats, despite their high sensitivity, were substantially more expensive (A$28.75 and A$57.08 per test, respectively). This study provides critical evidence to strengthen current honey bee biosecurity surveillance protocols and underscores the need for adoption of the most sensitive, standardized, cost-effective Tropilaelaps detection methods to improve early detection and thereby strengthen international honey bee biosecurity.

Indexed as

Mite InfestationsMitesAnimalsBeesDNA, EnvironmentalPapua New GuineaDNA, EnvironmentalApicultureBeekeepingEnvironmental DNA (eDNA)Honey bee biosecuritySurveillance methodsTropilaelaps mercedesae

Identifiers

PMID42143145
PMCPMC13376425

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.