Mechanism analysis of transgene expression induced by chitosan/hyaluronic acid nanoparticles in CD44v9/RHAMM co-expressed gastric cancer cells: contribution of hyaluronic acid to RHAMM-mediated intracellular transport.

Yu Torizuka, Shogo Nakamura, Hitoshi Tsugawa, Hidekazu Suzuki, Toshinori Sato

Abstract read

In one paragraph

Article in Glycoconjugate journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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5 · Who and what money

Authors and funding

5 authors.

Yu Torizuka *Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kouhoku, Yokohama, 223-8522, Japan.

Shogo Nakamura *Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kouhoku, Yokohama, 223-8522, Japan.

Hitoshi TsugawaTranskingdom Signaling Research Unit, Division of Host Defense Mechanism, Tokai University School of Medicine, 143 Shimokasuya, Isehara, 259-1193, Japan.

Hidekazu SuzukiDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, 259-1193, Japan.

Toshinori SatoDepartment of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kouhoku, Yokohama, 223-8522, Japan. sato@bio.keio.ac.jp.ORCID 0000-0002-4429-6101

Funding

JSPS KAKENHI 20H03667

6 · The paper itself

Abstract

Optimizing non-viral vectors for gene therapy requires elucidating the molecular mechanisms underlying vector uptake and intracellular delivery. In this study, cell transfection using pDNA/polysaccharide complexes was investigated to develop a gene delivery system specific for gastric tumor cells that express hyaluronic acid (HA) receptors such as CD44 variant 9 (CD44v9) and receptor for hyaluronan-mediated motility (RHAMM). pDNA/chitosan/HA complexes showed specific transgene expression activity in CD44v9(+) cells, mediated by CD44v9-dependent cell uptake and microtubule-dependent transport. Particularly, RHAMM was involved in intracellular transport of pDNA/chitosan/HA complexes. We demonstrated that HA drives CD44v9-mediated internalization, RHAMM-mediated, and microtubule-dependent intracellular transport, which are essential for enhanced pDNA complex-driven gene expression in gastric cancer cells co-expressing CD44v9 and RHAMM.

Indexed as

ChitosanExtracellular Matrix ProteinsHyaluronan ReceptorsHyaluronic AcidNanoparticlesStomach NeoplasmsTransgenesCell Line, TumorHumansTransfectionCD44 protein, humanChitosanExtracellular Matrix Proteinshyaluronan-mediated motility receptorHyaluronan ReceptorsHyaluronic AcidCD44v9ChitosanGene deliveryHyaluronic acidRHAMM

Identifiers

PMID42142272

DOI10.1007/s10719-026-10218-0

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.