Evidence map›Paper›PMID 42141726›Full record

ArticlePlant communications2026

CRISPR-mediated intronic knock-in of pre-amiRNA enables targeted gene silencing.

Jinsong Dong, Ke Zhang, Minglei Song, Menglan Xiao, Xianwei Lu, Shenghong Ge, Zai Zheng, Ming Li, Jian-Kang Zhu, Mingguang Lei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jinsong DongCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; Institute of Crop Sciences/National Nanfan Research Institute, Chinese Academy of Agricultural Sciences (CAAS), and Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Sanya 572024, China.
Ke ZhangShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Minglei SongInstitute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Menglan XiaoShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Xianwei LuCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Shenghong GeCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Zai ZhengNational Key Laboratory of Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology and Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan 570216, China.
Ming LiInstitute of Crop Sciences/National Nanfan Research Institute, Chinese Academy of Agricultural Sciences (CAAS), and Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Sanya 572024, China.
Jian-Kang ZhuInstitute of Crop Sciences/National Nanfan Research Institute, Chinese Academy of Agricultural Sciences (CAAS), and Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Sanya 572024, China; Institute of Advanced Biotechnology, Institute of Homeostatic Medicine, and School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China; Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China.
Mingguang LeiCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China. Electronic address: mglei@hznu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study introduces an intronic artificial microRNA (IamiRNA) strategy that combines CRISPR-Cas9-mediated knock-in with endogenous miRNA processing for targeted gene silencing in plants. By inserting amiRNA precursors into introns of endogenous genes, this approach enables effective, tissue-specific gene silencing without persistent transgene expression, offering a promising tool for functional genomics and crop improvement.

Indexed as

CRISPR-Cas SystemsGene Knock-In TechniquesGene SilencingIntronsMicroRNAsPlants, Genetically ModifiedMicroRNAs

Identifiers

PMID42141726
PMCPMC13589546

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.