A sensitive and cost-effective "turn-on" aptasensor for carcinoembryonic antigen using Ce-UiO-66 as an ultraefficient nanoquencher.

Mengqiu Li, Dongpo Xu, Wenfang Zhuang, Zien Cheng, Yiqian Sun, Jingxuan Qiu, Caiyun Liu, Jia Li, Xin Wang

Abstract read

In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Mengqiu LiSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Dongpo XuSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Wenfang ZhuangMedical Laboratory, Shidong Hospital Affiliated to University of Shanghai for Science and Technology, Shanghai, 200438, China.

Zien ChengMedical Laboratory, Shidong Hospital Affiliated to University of Shanghai for Science and Technology, Shanghai, 200438, China.

Yiqian SunNanshan College, Guangzhou Medical University, Guangzhou, 510182, China.

Jingxuan QiuSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Caiyun LiuSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Jia LiMedical Laboratory, Shidong Hospital Affiliated to University of Shanghai for Science and Technology, Shanghai, 200438, China.

Xin WangSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China. 18918629281@126.com.

Funding

National Natural Science Foundation of China 81773482the Research Innovation Team Project of Shidong Hospital, Yangpu District, Shanghai KYCX2510

6 · The paper itself

Abstract

Carcinoembryonic antigen (CEA) is a key tumor biomarker, and its sensitive detection is critical for early diagnosis and treatment monitoring. This work presents a novel "turn-on" fluorescent aptasensor for sensitive and cost-effective detection of CEA, based on a FAM-labeled CEA aptamer (FAM-Aptamer) as the recognition unit, and Ce-UiO-66 nanoparticles as an efficient quenching platform. The Ce-UiO-66 material was synthesized and comprehensively characterized by SEM, TEM, XPS, FT-IR, Raman and XRD, demonstrating a high quenching efficiency (QE) of 99.95% toward the fluorescent aptamer. This exceptional quenching performance is attributed to a synergistic mechanism involving FRET, PET, DQE and SQE. After systematic optimization of key parameters (Ce-UiO-66 concentration: 66.67 µg/mL; quenching time: 30 min; pH: 7.6; incubation temperature: 25 ℃; incubation time: 60 min), the aptasensor achieved a wide dual-linear response across 1-10 ng/mL and 10-100 ng/mL, respectively, with a detection limit of 13.78 pg/mL. Validation in diluted serum samples confirmed its accuracy and precision for CEA quantification, with recoveries of 95.50-109.60% and RSDs below 5.07%. The proposed aptasensor provides a reliable, cost-effective, and sensitive platform for CEA detection, serving as a proof-of-concept biosensing platform, with a certain potential for clinical application.

Indexed as

Aptamers, NucleotideBiosensing TechniquesCarcinoembryonic AntigenMetal NanoparticlesFluorescent Chemosensor CompoundsFluorescent DyesHumansLimit of DetectionAptamers, NucleotideCarcinoembryonic AntigenFluorescent Chemosensor CompoundsFluorescent DyesCarcinoembryonic antigenCe-UiO-66Fluorescent aptasensorSynergistic quenching

Identifiers

PMID42141320

DOI10.1007/s10876-023-02480-1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.