Evidence map›Paper›PMID 42141179›Full record

ArticleApplied microbiology and biotechnology2026

Synergistic attenuation of Aeromonas dhakensis virulence by dual deletion of PhoBR and KdpE two-component systems and its protective immunity in zebrafish.

Xuesong Li, Yukun Wu, Mengqi Wang, Roushan Li, Haibo Gao, Chenlu Yang, Aodi Yi, Xueqian Wang, Shuai Ye, Guiying Guo and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xuesong Li *Lab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Yukun Wu *Lab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Mengqi Wang *Lab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Roushan LiLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Haibo GaoDongfang Hewangxin Breeding Co., Ltd, Dongfang, 572600, China.
Chenlu YangLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Aodi YiLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Xueqian WangLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Shuai YeLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Guiying GuoSchool of Chemical Engineering and Technology, Hainan University, Haikou, 570228, China.
Lixia FanLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Nuo YangLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Jifeng ZengLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China.
Jiping ZhengLab of Microbiological Engineering (Infection and Immunity), School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, 570228, Hainan, China. zhenglab909@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aeromonas dhakensis, an emerging opportunistic pathogen prevalent in tropical regions, poses significant threats to both public health and aquaculture industries. This study elucidates the synergistic effects of dual deletion of the two-component systems PhoBR and KdpDE on virulence attenuation. Using homologous recombination and seamless cloning techniques, we constructed a ΔphoBRΔkdpE double mutant based on our laboratory's existing ΔphoBR mutant. Notably, phenotypic characterization revealed that the double mutant maintained comparable growth kinetics to wild-type, ΔphoBR mutant, and complemented strains under high-potassium conditions. However, under potassium-limiting conditions, ΔphoBRΔkdpE exhibited significantly impaired growth, compromised antioxidant capacity, diminished biofilm formation, reduced extracellular protease activity, and attenuated cell adhesion compared to controls. Furthermore, the mutant demonstrated enhanced susceptibility to antimicrobial peptides and marked virulence attenuation in zebrafish models, as evidenced by a substantially higher LD

Indexed as

AeromonasBacterial ProteinsBacterial VaccinesFish DiseasesGene DeletionGram-Negative Bacterial InfectionsAnimalsBiofilmsDisease Models, AnimalPotassiumVaccines, AttenuatedVirulenceVirulence FactorsZebrafishBacterial ProteinsBacterial VaccinesPotassiumVaccines, AttenuatedVirulence FactorsAeromonas dhakensisImmunityTwo-component systemsVirulence attenuationΔphoBRΔkdpE mutant strain

Identifiers

PMID42141179
PMCPMC13346194

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.