Evidence map›Paper›PMID 42138752›Full record

ArticleTransgenic research2026

Suppressor activity against cosuppression conferred by the petunia vein clearing virus genome.

Takumi Kitagawa, Hiroko Sobue, Kenta Oguri, Mashiro Yuhazu, Toshiyuki Fukuhara, Akira Kanazawa

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Takumi Kitagawa *Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-Ku, Sapporo, 060-8589, Japan.
Hiroko Sobue *Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-Ku, Sapporo, 060-8589, Japan.
Kenta OguriResearch Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-Ku, Sapporo, 060-8589, Japan.
Mashiro YuhazuResearch Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-Ku, Sapporo, 060-8589, Japan.
Toshiyuki FukuharaDepartment of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
Akira KanazawaResearch Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-Ku, Sapporo, 060-8589, Japan. kanazawa@agr.hokudai.ac.jp.

Funding

JSPS KAKENHI Grant number JP24K01727
6 · The paper itself

Abstract

The petunia genome contains an endogenous pararetrovirus, petunia vein clearing virus (PVCV). Previous analyses indicate that PVCV has suppressor activity against RNA silencing, but the suppressor protein has not been identified. Here we tested whether an open reading frame (ORF) of PVCV confers the activity that can suppress cosuppression of the CHS-A genes encoding chalcone synthase, which has a high rate of RNA turnover in the petal tissues of petunia. Petunia transformants that express PVCV ORF under the control of cauliflower mosaic virus 35S promoter were produced. The transgenic plants were crossed with those that have CHS-A cosuppression to produce plants that contain both the PVCV ORF transgene and CHS-A transgene. The coexistence of these transgenes resulted in phenotypic changes: pigmentation of various extents occurred on the originally white petals of CHS-A cosuppression phenotype. The generation of pigmented portions in flower petals coincided with higher transcript levels of CHS-A and PVCV ORF and less CHS-A short interfering RNA. These results indicate that the PVCV ORF can suppress CHS-A cosuppression and change the flower color phenotype when it is expressed as a transgene.

Indexed as

AcyltransferasesPetuniaPlants, Genetically ModifiedCaulimovirusFlowersGene Expression Regulation, PlantGenome, ViralOpen Reading FramesRNA InterferenceTransgenesAcyltransferasesflavanone synthetaseChalcone synthaseFlower color phenotypePararetrovirusRNA silencing suppressorTransgene

Identifiers

PMID42138752

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.