Evidence map›Paper›PMID 42138174›Full record

ArticleJournal of natural products2026

Proteotoxic Stress Bioreporter Enables Mechanism-Informed Antibiotic Discovery.

Julian Schubert, Christian Geibel, Anne Berscheid, Katharina W Wex, Giovanni Andrea Vitale, Chambers C Hughes, Daniel Petras, Heike Brötz-Oesterhelt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of natural products, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Julian SchubertDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0009-0005-9382-5945
Christian GeibelDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0009-0002-2044-9422
Anne BerscheidDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0003-1585-8715
Katharina W WexDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.
Giovanni Andrea VitaleDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0002-7143-6317
Chambers C HughesDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0002-7000-6438
Daniel PetrasCluster of Excellence EXC 2124: Controlling Microbes to Fight Infection (CMFI), University of Tuebingen, 72076 Tuebingen, Germany.
Heike Brötz-OesterheltDepartment of Microbial Bioactive Compounds, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tuebingen, 72076 Tuebingen, Germany.ORCID 0000-0001-9364-1832

Funding

Understanding the Impact of Xenobiotic Metabolism on the Gut Microbiome and Resilience against Gastrointestinal PathogensR35GM160154 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Daniel Petras · 2025 to 2026
$747k
NIGMS NIH HHS R35 GM160154
6 · The paper itself

Abstract

Antibiotic resistance poses a major global health challenge, which is intensified by the decline in antibiotic discovery efforts. This highlights the urgent need for innovative antibacterial agents. Natural products remain a key source of antibiotics, yet early mechanistic insights are often lacking. Classical whole-cell screening identifies growth inhibition but provides no mechanistic information. Bioreporter strains combine bioactivity detection with preliminary mechanistic profiling; however, they remain unavailable for several promising antibiotic target areas. We developed a sensitive bioreporter that signals the induction of the heat-shock Clp-ATPase gene

Indexed as

Anti-Bacterial AgentsBacillus subtilisBiological ProductsDrug DiscoveryMolecular StructureProteotoxic StressAnti-Bacterial AgentsBiological Products

Identifiers

PMID42138174
PMCPMC13316988

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.