ArticleFrontiers in veterinary science2026
Evaluation of multiple freeze-thaw cycles on growth factor and cytokine stability in canine platelet lysate: PDGF, TGF-
Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Platelet-rich plasma (PRP) and platelet lysate (PL) are biologically active products used in regenerative medicine because of their high concentrations of growth factors, cytokines, and other bioactive molecules that promote tissue repair and modulate inflammation. Freeze-thaw cycles are commonly used to activate PRP and induce growth factor release. While the optimal number of freeze-thaw cycles has been evaluated in human PRP, this parameter has not been investigated in canine PRP products. The objective of this study was to evaluate platelet counts and concentrations of platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF- Methods: Six vials of a commercially available canine PRP product from three separate manufacturing lots were evaluated. Each vial was reconstituted according to the manufacturer's instructions and stored at -80 °C for 12-24 h. Samples were thawed in a dry bath at 37 °C. Additional freeze-thaw cycles were performed using liquid nitrogen, with thawing at 37 °C for samples undergoing 3, 4, 5, and 10 cycles. A complete blood count was performed before freezing and after each freeze-thaw cycle. Concentrations of PDGF, TGF- Results: Initial platelet and white blood cell counts were consistent with manufacturer-reported values. Platelet counts were significantly reduced following 4, 5, and 10 freeze-thaw cycles compared with initial PRP values. Concentrations of PDGF, TGF- Discussion: Four to five freeze-thaw cycles effectively lysed platelets while preserving PDGF and TGF-
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