Evidence map›Paper›PMID 42137602›Full record

ArticleMolecular therapy. Advances2026

Rapid multiplex liver gene-editing in mice using adeno-associated virus 8 or lipid nanoparticles.

Dandan Wu, Isabelle Bolt, Dagmar W Tolenaars, Suzanne Duijst, Wietse In Het Panhuis, Stijn R J Hofstraat, Robby Zwolsman, Roy van der Meel, Coen C Paulusma, Stan F J van de Graaf

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Article in Molecular therapy. Advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dandan WuTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Isabelle BoltTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Dagmar W TolenaarsTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Suzanne DuijstTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Wietse In Het PanhuisTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Stijn R J HofstraatLaboratory of Chemical Biology, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, the Netherlands.
Robby ZwolsmanLaboratory of Chemical Biology, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, the Netherlands.
Roy van der MeelLaboratory of Chemical Biology, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, the Netherlands.
Coen C PaulusmaTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.
Stan F J van de GraafTytgat Institute for Liver and Intestinal Research, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 69, 1105 BK Amsterdam, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Somatic liver knockout (SLiK) is a method developed to rapidly generate liver-specific knockout of one or several genes. However, the original protocol relies on hydrodynamic tail vein injection (HTVI), a procedure associated with low transfection efficiency and animal discomfort due to cardiac stress. To address these challenges, we evaluated whether HTVI could be adapted to adeno-associated virus serotype 8 (AAV8) or lipid nanoparticles (LNPs). In addition, we sought to expand SLiK functionality by incorporating transgene overexpression together with gene knockout. Two AAV8 vectors were co-injected into

Indexed as

bile salt conjugationcholestasismore humanized mice modelrapid gene-editingtyrosinemia

Identifiers

PMID42137602
PMCPMC13148919

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.