Evidence map›Paper›PMID 42135952›Full record

ArticleJournal of animal science2026

Low-dose ozone application during storage modulates yolk oxidation and enhances hatchability in broiler hatching eggs.

Sinan Vicil, Fatma Tülin Özbaşer Bulut, Metin Bayraktar, Muazzez Gürgan Eser

Abstract read
In one paragraph

Article in Journal of animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sinan VicilFaculty of Veterinary Medicine, Department of Biochemistry, Tekirdag Namik Kemal University, Tekirdag, 59030, Turkey.
Fatma Tülin Özbaşer BulutFaculty of Veterinary Medicine, Department of Animal Science, Tekirdag Namik Kemal University, Tekirdag, 59030, Turkey.
Metin BayraktarFaculty of Veterinary Medicine, Department of Animal Science, Tekirdag Namik Kemal University, Tekirdag, 59030, Turkey.
Muazzez Gürgan EserFaculty of Arts and Sciences, Department of Biology, Tekirdag Namik Kemal University, Tekirdag, 59030, Turkey.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated whether low-dose ozone exposure during storage can mitigate the negative effects of long-term storage on egg quality, yolk oxidative status, and hatching outcomes in broiler hatching eggs. A total of 1,350 eggs from 67-wk-old Ross 308 breeders were allocated to a 2 × 3 factorial arrangement of treatments: storage for 0 or 14 d and daily exposure to 0, 4, or 7 ppm ozone for 30 min. Egg quality traits (albumen index, yolk index, Haugh unit, shell thickness and ratios), eggshell microbial load (log10 CFU/mL), yolk malondialdehyde (MDA; nmol/mg protein), yolk protein content (mg/mL), and hatchability and embryonic mortality were recorded. Data were analyzed by two-way ANOVA with storage time and ozone dose as fixed effects. Fourteen days of storage reduced albumen index, yolk index, and Haugh unit and increased yolk MDA (5.70 vs. 8.87 nmol/mg protein; P < 0.001) and protein content (14.34 vs. 15.24 mg/mL; P  < 0.01). Ozone markedly reduced eggshell microbial load in non-stored and stored eggs (eg in stored eggs, 9.14, 3.69, and 1.77 log10 CFU/mL at 0, 4, and 7 ppm; P  = 0.001). Yolk MDA was greater at 7 ppm than at 0 or 4 ppm at both storage times (P  ≤ 0.002), whereas 4 ppm did not differ from 0 ppm. For eggs stored, 0 d, 4 ppm increased hatchability of fertile eggs (96.42%) compared with 0 ppm (91.07%; P  = 0.034). After 14 d of storage, 4 ppm resulted in greater hatchability of fertile eggs than 7 ppm (92.92 vs. 80.39%; P  = 0.025), whereas values for the 0 ppm group (86.45%) were intermediate and did not differ from either group; 4 ppm also reduced early embryonic mortality compared with 7 ppm (2.65 vs. 11.76%; P  = 0.007). In conclusion, low-dose ozone (4 ppm) applied during storage reduced eggshell microbial load and supported embryonic viability and hatchability without increasing yolk lipid peroxidation, whereas higher ozone (7 ppm) increased yolk MDA and early embryonic loss in long-stored eggs.

Indexed as

ChickensEgg YolkFood StorageOvumOzoneAnimalsDose-Response Relationship, DrugFemaleOxidation-ReductionOzonebroiler breederegg qualityhatchabilitymalondialdehydeozonestorage

Identifiers

PMID42135952
PMCPMC13228150

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.