ArticleBiotechnology for biofuels and bioproducts2026
Process optimisation for polyhydroxyalkanoates production with tailored hydroxyvalerate content from agri-food residues using mixed microbial cultures.
Article in Biotechnology for biofuels and bioproducts, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Upcycling agricultural residues into polyhydroxyalkanoates through pilot-scale mixed microbial culture processes.Frontiers in bioengineering and biotechnology · 2026Article
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10 authors.
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Abstract
backgroundPolyhydroxyalkanoates (PHAs) are promising bio-based and biodegradable polymers that can contribute to the transition towards a circular bioeconomy, particularly when produced from renewable feedstocks. Nevertheless, the use of mixed microbial cultures (MMC) and real agri-food residues poses challenges related to substrate variability, process stability and control of polymer composition. In this study, a three-stage process was investigated to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from agri-food residues. The process consisted of: (i) acidogenic fermentation of cattle slurry and corn residues to generate a carboxylic acid (CA)-rich stream; (ii) microbial selection under feast-famine conditions to enrich PHA-accumulating microorganisms; and (iii) PHA accumulation in a fed-batch reactor. Two operational setups, differing in the pretreatment type, were compared to evaluate possible effects on PHA production: setup 1 used centrifuged fermentate, whereas setup 2 included an additional 0.22-µm filtration step.
resultsAcidogenic fermentation produced up to 18.6 gCOD/L of CAs, mainly acetic, butyric and propionic acids, with production strongly influenced by substrate ratio, hydraulic retention time and pH. The CA-rich fermented liquid was used to select MMC enriched with PHBV-accumulating microorganisms and to tune the PHBV composition during the subsequent accumulation phase. In setup 1, storage yield (mean 0.33 ± 0.23 gCOD
conclusionsOverall, the results indicate that MMC-based PHBV production from agri-food residues can achieve polymer contents comparable to those reported for similar systems using complex substrates, while highlighting the importance of feedstock pretreatment and process control. The study provides insights into the integration of acidogenic fermentation with MMC-based PHA production, and identifies operational aspects that require further optimisation to enhance process robustness and product consistency.
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