Evidence map›Paper›PMID 42135832›Full record

ArticleJournal of nanobiotechnology2026

A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons.

Rashi Goel, Kristina Jevdokimenko, Ronja Rehm, Jannik Hentze, Paola Agüi-Gonzalez, Momchil Ninov, Erik Maier, Yin Wu, Felix Lange, Agata Witkowska and 32 more

Abstract read
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In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

42 authors.

Rashi GoelLaboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Kristina JevdokimenkoInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Ronja RehmInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Jannik HentzeInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Paola Agüi-GonzalezInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Momchil NinovBioanalytical Mass Spectrometry Research Group, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Erik MaierInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Yin WuInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Felix LangeDepartment of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Agata WitkowskaLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125, Berlin, Germany.
Svenja BolzLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125, Berlin, Germany.
Francesca PennacchiettiDepartment of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, 17165, Sweden.
Martina DamentiDepartment of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, 17165, Sweden.
Natalie Kaempf8 VIB-KU Leuven Center for Neuroscience, Leuven, 3000, Belgium.
Vladimir KhayenkoRudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, 97080, Wuerzburg, Germany.
Carles Calatayud8 VIB-KU Leuven Center for Neuroscience, Leuven, 3000, Belgium.
Viveka Nand MalviyaLaboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Natali L ChanadayDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Emma Scaletti HutchinsonDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden.
Hao LiuDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden.
Kirsten WeyandDepartment of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany.
Valentin SchwarzeDepartment of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany.
Daniela IvanovaCentre for Discovery Brain Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, Scotland, UK.
Tristan P WallisClem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia.
Christopher SmallClem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia.
Hans M MaricRudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, 97080, Wuerzburg, Germany.
Merja JoensuuClem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia.
Michael A CousinCentre for Discovery Brain Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, Scotland, UK.
Frédéric A MeunierClem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia.
Patrik Verstreken8 VIB-KU Leuven Center for Neuroscience, Leuven, 3000, Belgium.
Ilaria TestaDepartment of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, 17165, Sweden.
Ege T KavalaliDepartment of Pharmacology, School of Medicine, Vanderbilt University, Nashville, TN, 37240-7933, USA.
Volker HauckeLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125, Berlin, Germany.
Stefan JakobsDepartment of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Henning UrlaubBioanalytical Mass Spectrometry Research Group, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Nils BroseDepartment of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany.
Benjamin H CooperDepartment of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37075, Göttingen, Germany.
Pål StenmarkDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, SE-106 91, Sweden.
Felipe OpazoInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Reinhard JahnLaboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany.
Silvio O RizzoliInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.
Eugenio F FornasieroInstitute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany. efornas@gwdg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes affected in neural diseases. Monitoring this process, particularly synaptic vesicle recycling, in living cells has been instrumental in revealing the mechanisms responsible for neurotransmitter release. However, currently available reporters suffer from limitations, such as large probe sizes or limited compatibility for human neurons, hampering the quantitative analysis of synaptic pathophysiology. Here, we describe the NbLumSyt1 toolkit, a panel of nanobody-based affinity probes that target the luminal domain of the synaptic vesicle protein Synaptotagmin 1 (Syt1). These new tools enable quantitative, noninvasive imaging and functional interrogation of Syt1 exo-endocytosis and trafficking in human neurons, with unprecedented precision, versatility and cost efficiency, in technologies ranging from fixed- and live-cell super-resolution imaging to electron microscopy and mass spectrometry. Overall, NbLumSyt1 nanobinders provide a valuable platform for studying synaptic physiology and pathophysiology, benefiting fundamental neuroscience and translational efforts to study and develop treatments for brain-related disorders.

Indexed as

Neuronal PlasticityNeuronsSingle-Domain AntibodiesSynaptic VesiclesAnimalsEndocytosisHumansMiceRatsSynaptic TransmissionSynaptotagmin ISingle-Domain AntibodiesSynaptotagmin IBiomedical engineeringNanobodiesNanotoolsPresynaptic biologySdAbSynaptic vesiclesSynaptotagmin

Identifiers

PMID42135832
PMCPMC13185310

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.