Exploiting the ability of Bacillus subtilis to synthetize surfactin under oxygen limitation.

Eric Hiller, Lea Rahel Tadele, Nikolina Miceta, Dominik Notz, Elvio Henrique Benatto Perino, Rudolf Hausmann

Abstract read

In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eric HillerDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany. eric.hiller@uni-hohenheim.de.ORCID http://orcid.org/0009-0000-3815-920X

Lea Rahel TadeleDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.ORCID http://orcid.org/0009-0004-8170-572X

Nikolina MicetaDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.ORCID http://orcid.org/0009-0004-4614-8462

Dominik NotzDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.ORCID http://orcid.org/0009-0000-3852-8781

Elvio Henrique Benatto PerinoDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.ORCID http://orcid.org/0000-0003-0372-260X

Rudolf HausmannDepartment of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.ORCID http://orcid.org/0000-0002-2327-7120

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surfactin production by Bacillus subtilis is typically performed under aerobic conditions, requiring high aeration and agitation, which leads to mechanical stress for the cells and therefore promotes excessive foaming. As a process-oriented alternative to full aerobic operation, an aerobic to micro-aerobic switching strategy was developed that aims to decouple biomass formation from surfactin synthesis by controlling oxygen availability. Promoter activities relevant to nitrate respiration and surfactin biosynthesis were first characterised in shake flasks using transcriptional reporter strains and online monitoring of dissolved oxygen. The nitrite reductase promoter showed the strongest induction under oxygen-limited conditions and was used to construct an oxygen-responsive production strain in which the native P

Indexed as

Bacillus subtilisLipopeptidesOxygenPeptides, CyclicAerobiosisBiomassBioreactorsBiosurfactantsFermentationGene Expression Regulation, BacterialGlucoseMetabolic EngineeringNitratesPromoter Regions, GeneticGlucoseLipopeptidesNitratesOxygenPeptides, Cyclicsurfactin peptideBioreactor scale-upFed-batch fermentationLipopeptide productionNitrogen metabolismOxygen-limited cultivationProcess kinetics

Identifiers

PMID42135565

DOI10.1007/s00253-026-13868-0

PMCPMC13183733

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.