Evidence map›Paper›PMID 42135295›Full record

ArticleNature communications2026

Using rotational integration of oblique interferometric scattering to track axial spatiotemporal responses of tubular membrane protrusions.

Junyu Liu, Yean Jin Lim, David Herrmann, Paul Timpson, Tri Giang Phan, Viviane Delghingaro-Augusto, Christopher Richard Parish, Huafeng Liu, Min Guo, Woei Ming Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Junyu LiuState Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.ORCID 0000-0003-4276-9531
Yean Jin LimDivision of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0003-1874-2662
David HerrmannCancer Ecosystems Program, Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Darlinghurst, NSW, Australia.ORCID 0000-0002-9514-7501
Paul TimpsonCancer Ecosystems Program, Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Darlinghurst, NSW, Australia.ORCID 0000-0002-5514-7080
Tri Giang PhanPrecision Immunology Program, Garvan Institute of Medical Research, Sydney, NSW, Australia.ORCID 0000-0002-4909-2984
Viviane Delghingaro-AugustoDivision of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0002-5107-2384
Christopher Richard ParishDivision of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0001-7740-0430
Huafeng LiuState Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China. liuhf@zju.edu.cn.
Min GuoState Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China. guom@zju.edu.cn.ORCID 0000-0002-2093-8771
Woei Ming LeeDivision of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia. steve.lee@anu.edu.au.ORCID 0000-0002-3912-6095

Funding

National Natural Science Foundation of China (National Science Foundation of China) 62427807National Natural Science Foundation of China (National Science Foundation of China) 62475232
6 · The paper itself

Abstract

The 3D spatiotemporal dynamics of tubular membrane protrusions are crucial for understanding phagocytosis, cellular communication and mechanobiology. Confocal microscopy, despite its prevalent use in membrane protrusion studies, presents limitations due to its inherently low axial resolution and high phototoxicity, which significantly hinder live imaging of tubular protrusion along the axial plane. We discovered that rotational oblique interference scattering (RO-iSCAT) leverages off-axis illumination to induce a larger lateral shift in out-of-focus iSCAT signals compared to in-focus signals. This phenomenon generates speckle-free widefield interferometric scattering signals with a 10-fold signal-to-noise ratio improvement, eliminating the need for any background subtraction. RO-iSCAT enables real-time, label-free imaging of diverse nanoparticles and tubular membrane protrusions, thus providing biophysical profiling of tubular membrane protrusions across multiple cell types and in complex co-cultures. RO-iSCAT empowers rapid quantitative dissection of the axial spatiotemporal complexities of membrane protrusions at tens to hundreds of nanometer displacements without requiring 3D volumetric imaging.

Indexed as

Cell Surface ExtensionsInterferometryAnimalsCell MembraneHumansImaging, Three-DimensionalMicroscopy, ConfocalMicroscopy, InterferenceRotation

Identifiers

PMID42135295
PMCPMC13176345

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.