Evidence map›Paper›PMID 42134445›Full record

ArticleExperimental eye research2026

YAP1-dependent regulation of cell size in BCAM-positive limbal corneal progenitor cells.

Kosei Suzuki, Shinri Sato, Markus H Frank, Natasha Y Frank, Yuzuru Sasamoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Kosei SuzukiDepartment of Ophthalmology, University of Washington, Seattle, WA, USA.
Shinri SatoDepartment of Ophthalmology, University of Washington, Seattle, WA, USA.
Markus H FrankTransplant Research Program, Boston Children's Hospital, Boston, MA, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA; Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women's Hospital, Boston, MA, USA; School of Medical and Health Sciences, Edith Cowan University, Perth, Western Australia, Australia.
Natasha Y FrankHarvard Stem Cell Institute, Harvard University, Cambridge, MA, USA; Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Department of Medicine, VA Boston Healthcare System, Boston, MA, USA. Electronic address: nyfrank@bwh.harvard.edu.
Yuzuru SasamotoDepartment of Ophthalmology, University of Washington, Seattle, WA, USA; Transplant Research Program, Boston Children's Hospital, Boston, MA, USA; Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA. Electronic address: ysasamot@uw.edu.

Funding

The role of BCAM-positive cells in central cornea, limbus and conjunctivaR01EY036399 · NEI · UNIVERSITY OF WASHINGTON · PI Yuzuru Sasamoto · 2024 to 2026
$2.3M
Development of cell-free approaches to the treatment of limbal stem cell deficiencyR00EY031741 · NEI · UNIVERSITY OF WASHINGTON · PI SASAMOTO, YUZURU · 2023 to 2025
$689k
The role of limbal fibroblasts for the maintenance of limbal stem cellsR21EY035735 · NEI · UNIVERSITY OF WASHINGTON · PI SASAMOTO, YUZURU · 2024 to 2025
$468k
Development of cell-free approaches to the treatment of limbal stem cell deficiencyK99EY031741 · NEI · BRIGHAM AND WOMEN'S HOSPITAL · PI SASAMOTO, YUZURU · 2020 to 2021
$220k
NEI NIH HHS K99 EY031741NEI NIH HHS R00 EY031741NEI NIH HHS R01 EY036399NEI NIH HHS R21 EY035735
6 · The paper itself

Abstract

Cell size has been associated with stem and progenitor cell states across multiple tissues, yet its regulation in the human corneal epithelium remains incompletely understood. This study aimed to characterize cell-size differences among limbal and corneal epithelial cell populations defined by the limbal stem cell (LSC) marker ABCB5 and the transit-amplifying cell (TAC) marker BCAM, and to investigate the molecular regulators of progenitor cell size. ABCB5-positive LSCs and BCAM-positive TACs were identified and isolated by flow cytometry, and the mean cell size was estimated using forward scatter (FSC). As a result, ABCB5-positive LSCs were significantly smaller than ABCB5-negative limbal epithelial cells, and BCAM-positive TACs exhibited a smaller cell size than BCAM-negative cells, particularly in the limbus. Immunofluorescence staining showed that Yes-associated protein 1 (YAP1) and BCAM were co-expressed in basal epithelial cells in the human limbus and cornea. Expression of the YAP1 transcriptional target gene CYR61 was elevated in BCAM-positive limbal cells. SiRNA-mediated knockdown of YAP1 or BCAM in cultured human limbal epithelial cells resulted in a significant increase in cell size. These findings identify small cell size as a characteristic feature of limbal BCAM-positive TACs and demonstrate that progenitor cell size is regulated by YAP1 and BCAM, highlighting an interaction between physical cell properties and progenitor function in the corneal epithelium.

Indexed as

Adaptor Proteins, Signal TransducingEpithelium, CornealGene Expression RegulationLimbus CorneaePhosphoproteinsStem CellsCells, CulturedCell SizeFlow CytometryHumansLimbal Stem CellsTranscription FactorsYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingPhosphoproteinsTranscription FactorsYAP1 protein, humanYAP-Signaling ProteinsABCB5BCAMCell sizeLimbal stem cellTransit-amplifying cellsYAP1

Identifiers

PMID42134445
PMCPMC13286696

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.