ArticleJournal of the American Academy of Dermatology2026
Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.
Article in Journal of the American Academy of Dermatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundMohs surgery has excellent outcomes but is time-consuming due to the use of intraoperative frozen sections. Two-photon fluorescence microscopy (TPFM) is a slide-free technique that generates H&E-like histology images in fresh tissue specimens in real-time which could accelerate treatment.
objectivesTo determine the accuracy of TPFM with H&E coloring for the evaluation of basal cell carcinoma on Mohs specimens.
methodsFrozen section analysis and TPFM images were acquired from 133 specimens following completion of Mohs surgery for basal cell carcinoma. Two Mohs surgeons who had not previously seen TPFM histology evaluated the TPFM images. Their evaluations (TPFM read) were compared to a consensus frozen section analysis (frozen section analysis read) graded by a dermatopathologist and separate Mohs surgeon.
resultsThe average sensitivity and specificity were 93.8% and 82.2%, respectively. If specimens that were discordant due to section plane differences or tissue fragments from frozen sectioning are excluded, average sensitivity and specificity improve to 95.0% and 94.3%, respectively, which is a truer measure of the diagnostic accuracy. LIMITATIONS: This study evaluated agreement between histology after conclusion of surgery.
conclusionMohs surgeons can interpret H&E colored TPFM images with comparable accuracy to frozen sections on Mohs margins taken for basal cell carcinoma with minimal training.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.