Protocol for covalent RNA labeling by RiboLight dyes for detection by in-gel fluorescence and fluorescence microscopy.

Moon Jung Kim, Jinwoo Shin, Eric T Kool

Erratum issuedAbstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Erratum issued
Protocol for covalent RNA labeling by RiboLight dyes for detection by in-gel fluorescence and fluorescence microscopy.2026

5 · Who and what money

Authors and funding

3 authors.

Moon Jung KimDepartment of Chemistry and Stanford Cancer Institute, Stanford University, Stanford, CA 94305, USA.

Jinwoo ShinDepartment of Chemistry and Stanford Cancer Institute, Stanford University, Stanford, CA 94305, USA.

Eric T KoolDepartment of Chemistry and Stanford Cancer Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: kool@stanford.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA abundance and localization provide key insights into cellular physiology and disease, making RNA a central target of basic and applied research. Here, we present a protocol for covalent labeling of RNA using RiboLight dyes for detection in gels and cells. We outline steps for cell culture, in vitro and cellular RNA labeling, sample purification, and visualization by in-gel fluorescence and fluorescence microscopy. This approach integrates readily into molecular and cellular biology workflows for RNA analysis. For complete details on the use and execution of this protocol, please refer to Shin et al.

Indexed as

Fluorescent DyesRNAStaining and LabelingHumansMicroscopy, FluorescenceFluorescent DyesRNAChemistryMicroscopyMolecular/Chemical Probes

Identifiers

PMID42133491

DOI10.1016/j.xpro.2026.104579

PMCPMC13196512

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.