Evidence map›Paper›PMID 42133488›Full record

ArticleSTAR protocols2026

Protocol for intracardiac delivery and live imaging of NK-tumor interactions in an ex ovo chick embryo model.

Mira Saari, Emmi Järvelä, Talha Ali, Hamza Siddiqui, Helka Göös, Matti Korhonen, Diana Schenkwein, Seppo Ylä-Herttuala, Seppo Vainio, Ilya Skovorodkin and 1 more

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mira SaariICell, Research and Development, Finnish Red Cross Blood Service, 00290 Helsinki, Finland; A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70211 Kuopio, Finland.
Emmi JärveläICell, Research and Development, Finnish Red Cross Blood Service, 00290 Helsinki, Finland; A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70211 Kuopio, Finland.
Talha AliCenter for Machine Vision and Signal Analysis (CMVS) University of Oulu, Pentti Kaiteran Katu 1, 90014 Oulu, Finland.
Hamza SiddiquiLaboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5A, 90014 Oulu, Finland.
Helka GöösICell, Research and Development, Finnish Red Cross Blood Service, 00290 Helsinki, Finland.
Matti KorhonenICell, Research and Development, Finnish Red Cross Blood Service, 00290 Helsinki, Finland.
Diana SchenkweinA. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70211 Kuopio, Finland.
Seppo Ylä-HerttualaA. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 70211 Kuopio, Finland.
Seppo VainioLaboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5A, 90014 Oulu, Finland.
Ilya SkovorodkinLaboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5A, 90014 Oulu, Finland. Electronic address: ilya.skovorodkin@oulu.fi.
Farhana JahanICell, Research and Development, Finnish Red Cross Blood Service, 00290 Helsinki, Finland. Electronic address: farhana.jahan@bloodservice.fi.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a protocol to evaluate natural killer (NK)-tumor interactions using an ex ovo chick embryo system. We describe procedures to culture GFP-labeled SH-SY5Y neuroblastoma cells, expand and Hoechst-label human NK cells, and perform sequential intracardiac injections. We use fluorescence imaging to visualize NK and tumor cell distribution in vivo and live imaging to assess interactions in vitro. This approach provides a rapid and accessible platform to quantify NK cell activity in a living vertebrate model.

Indexed as

Killer Cells, NaturalAnimalsCell Line, TumorChick EmbryoHumansCancerImmunologyMicroscopyModel Organisms

Identifiers

PMID42133488
PMCPMC13196521

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.