Evidence map›Paper›PMID 42132931›Full record

ArticleThe Journal of general virology2026

Establishment of a human coronavirus HKU1 infection evaluation system using apical-out airway organoids.

Masatoshi Kakizaki, Satoko Sugimoto, Miyuki Kawase, Sakurako Norito, Hisao Okabe, Hayato Go, Koichi Hashimoto, Kazuya Shirato

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Masatoshi KakizakiDepartment of Respiratory Viruses, National Institute of Infectious Diseases, Japan Institute for Health Security, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Satoko SugimotoDepartment of Respiratory Viruses, National Institute of Infectious Diseases, Japan Institute for Health Security, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Miyuki KawaseDepartment of Respiratory Viruses, National Institute of Infectious Diseases, Japan Institute for Health Security, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Sakurako NoritoDepartment of Pediatrics, School of Medicine, Fukushima Medical University, 1st Hikarigaoka, Fukushima, Fukushima 960-1295, Japan.
Hisao OkabeDepartment of Pediatrics, School of Medicine, Fukushima Medical University, 1st Hikarigaoka, Fukushima, Fukushima 960-1295, Japan.
Hayato GoDepartment of Pediatrics, School of Medicine, Fukushima Medical University, 1st Hikarigaoka, Fukushima, Fukushima 960-1295, Japan.
Koichi HashimotoDepartment of Pediatrics, School of Medicine, Fukushima Medical University, 1st Hikarigaoka, Fukushima, Fukushima 960-1295, Japan.
Kazuya ShiratoDepartment of Respiratory Viruses, National Institute of Infectious Diseases, Japan Institute for Health Security, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human coronavirus HKU1 (HCoV-HKU1) is difficult to study because it does not propagate in standard cell lines. Consequently, research has relied on the air-liquid interface model, which is limited by long culture periods and the absence of cytopathic effects (CPEs). To address these challenges, we established an infection evaluation system using apical-out airway organoids (AOAOs). Derived from human bronchial epithelial cells, AOAOs supported efficient HCoV-HKU1 replication, and immunofluorescence revealed a preferential infection of ciliated cells. Notably, HCoV-HKU1 induced a distinct CPE characterized by organoid dissociation, enabling the quantification of viral titres via the TCID

Indexed as

CoronavirusCoronavirus InfectionsOrganoidsAntiviral AgentsBenzamidinesCell LineCytopathogenic Effect, ViralEpithelial CellsGuanidinesHumansVirus ReplicationAntiviral AgentsBenzamidinesGuanidinesnafamostatair–liquid interface cultureapical-out airway organoidscytopathic effecthuman coronavirus HKU1 (HCoV-HKU1)

Identifiers

PMID42132931
PMCPMC13175505

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.