ArticleJournal of virology2026
Generation of HBV cccDNA using single-stranded M13 phage DNA for authentic minichromosome functionality.
Yumeng Li, Ting Hua, Menghan Hao, Liman Chen, Wenjun Huang, Zhong Fang, Asha Ashuo, Yaming Li, Zhigang Yi, Hongzhou Gu and 2 more
Abstract read
In one paragraphArticle in Journal of virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
12 authors.
Yumeng Li *MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.ORCID 0009-0002-4718-3775 Ting Hua *MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.
Menghan HaoMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.
Liman ChenInstitutes of Biomedical Sciences, Fudan University, Shanghai, China.
Wenjun HuangMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.
Zhong FangMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.ORCID 0000-0002-7703-8686 Asha AshuoMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.
Yaming LiMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.
Zhigang YiMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.ORCID 0000-0002-4560-4970 Zhenghong YuanMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.ORCID 0000-0003-0268-4891 Jieliang ChenMOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai, China.ORCID 0000-0002-9647-0239 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) persists as minichromosomes in the nuclei of hepatocytes and serves as the transcriptional template for viral RNAs. The stable cccDNA pool, characterized by a very low copy number, poses challenges for studying its molecular characteristics and developing targeted therapeutics. To accurately mimic the full-length sequences and structure of HBV cccDNA, we developed a novel method using an M13 phage-based system and deoxyribozyme to produce full-length single-stranded DNA (ssDNA) corresponding to both the positive and negative strands of cccDNA. This method involves the cyclization of the ssDNA followed by the addition of T4 DNA ligase, thereby producing the cccDNA. The IMPORTANCE: HBV remains a major global health burden, with cccDNA serving as the transcriptional template for viral RNAs that sustain persistent infection. The low copy number of cccDNA in the nucleus and the lack of authentic
Indexed as
Bacteriophage M13DNA, CircularDNA, Single-StrandedDNA, ViralHepatitis B virusExtrachromosomal DNAHepatocytesHumansRNA, ViralVirus ReplicationDNA, CircularDNA, Single-StrandedDNA, ViralExtrachromosomal DNARNA, Viralantiviralscovalently closed circular DNAhepatitis B virusminichromosomessDNA
Identifiers
PMID42132423
PMCPMC13288475
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