ArticleFrontiers in microbiology2026
From soil to crop: a tiered screening strategy for lipopeptide-producing
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: To address agrochemical pollution and improve microbial screening efficiency, this study aimed to develop an integrated pipeline for isolating high-yielding lipopeptide-producing Methods: A tiered screening strategy combining hemolytic activity assay, plate confrontation, and oil-spreading assay was established to isolate strains from rhizosphere soils. Lipopeptide production was identified by MALDI-TOF MS and quantified via HPLC. Genomic analysis was performed to elucidate the genetic basis of lipopeptide synthesis. Crude lipopeptide extracts were evaluated for antimicrobial activity via plate assays, and for plant growth-promoting effects under pot and field conditions. Results: The screening successfully identified potent Discussion: This study provides an integrated pipeline from strain screening to field validation, highlighting Bacillus lipopeptides as sustainable alternatives to agrochemicals for combined biocontrol and plant growth promotion.
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