ArticleJournal of animal science and biotechnology2026
Transcriptome profiling of bovine preantral follicles during early folliculogenesis.
Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 1 synthesis or guideline pooled it.
- Dynamic Remodeling of Oocyte-Granulosa Cell Communication During Bovine Folliculogenesis Revealed by Transcriptomic Meta-Analyses.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Pooled it
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundThe ovarian reserve, primarily composed of primordial (PMF), primary (PF), and secondary follicles (SF), holds great potential for fertility preservation, yet remains inaccessible mainly due to the challenges of replicating early folliculogenesis in vitro. The molecular mechanisms governing the activation and differentiation of oocytes from the dormant PMF pool remain poorly defined. In this study, we used a bovine model to investigate early follicular development.
resultsThe transcriptome of isolated PMF, PF, and SF revealed different profiles of the follicles during early folliculogenesis. Differential gene expression analyses between PF versus PMF and SF versus PF revealed 689 and 3,206 significantly regulated genes, respectively (FDR < 0.05), highlighting key pathways including PI3K-Akt, Wnt, mTOR, and ECM-receptor interaction. Between PF versus PMF and SF versus PF, 13 and 69 DEGs were identified as transcription factors, respectively. A Likelihood Ratio Test followed by hierarchical clustering identified four major gene expression clusters across the three stages, showing significant enrichment in processes such as ECM-related functions and cell cycle. Based on the enrichment of the cell cycle pathway, we focused on RAD51, which was highly expressed in preantral follicles. Immunohistochemical analysis further showed that RAD51 protein localized in the ooplasm, along with the mitochondrial marker HSP60.
conclusionThis is the first study to perform bulk RNA sequencing on isolated preantral follicles (PMF, PF, and SF) in cattle. Our findings provide new insights into the stage-specific mechanisms regulating follicle activation and growth in cattle, laying the groundwork for future in vitro fertility preservation strategies in both clinical and conservation contexts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.