Evidence map›Paper›PMID 42129278›Full record

ArticleScientific reports2026

Genome-wide identification and expression analysis of the NBS-LRR gene family in Citrus clementina.

Min Wu, Meizhi Lin, Qiuyun Gan, Guiwei Cai, Chentai Jin, Yingqi Liu, Ping Wang, Xiaokang Zhuo

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Min WuSchool of Computing and information Science, Fuzhou Institute of Technology, Lianjiang, 350506, Fujian, China.
Meizhi LinSchool of Computing and information Science, Fuzhou Institute of Technology, Lianjiang, 350506, Fujian, China.
Qiuyun GanSchool of Computing and information Science, Fuzhou Institute of Technology, Lianjiang, 350506, Fujian, China.
Guiwei CaiSchool of Computing and information Science, Fuzhou Institute of Technology, Lianjiang, 350506, Fujian, China.
Chentai JinKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yingqi LiuKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Ping WangKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. wangp0518@fafu.edu.cn.
Xiaokang ZhuoKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. zhuoxiaokang@fafu.edu.cn.

Funding

the earmarked fund for China Agriculture Research System CARS-26the Fundamental Research Funds for the Central Universities BFUKF202409
6 · The paper itself

Abstract

Citrus is a highly important global fruit crop that faces significant challenges from citrus Huanglongbing (HLB). The nucleotide binding site leucine-rich repeat (NBS-LRR) gene family plays a central role in plant immunity. However, comprehensive studies on the function of NBS-LRR genes in HLB resistance or tolerance remain limited. In this study, we identified 278 NBS-LRR genes from Citrus clementina. These genes exhibit 7 domain architectures. Physicochemical analyses revealed significant subfamily divergence in length (91-2101 aa), molecular weight (10.57-237.18 kDa), and isoelectric point (pI, 4.61-9.35). Over 82% of proteins localize to the nucleus/cytoplasm, indicating roles in signal transduction and transcriptional regulation. NBS-LRR genes cluster non-randomly among chromosomes, forming tandem repeats on chromosomes 3, 5, and 7. Phylogenetic analysis grouped the NBS-LRR family into 5 clades. Conserved domain analysis identified 10 motifs; Motif 1 and 2 are highly conserved, potentially involved in pathogen recognition. Promoter analysis detected 36 cis-acting elements linked to diverse processes (e.g., hormone/stress response). Synteny analysis revealed 9 genes are orthologous genes of Arabidopsis thaliana. Crucially, transcriptome data showed that significantly higher NBS-LRR expression in Huanglongbing-infected plants, pinpointing candidate gene Ciclev10018143m.1. This study provides a foundation for citrus disease resistance breeding and candidate genes for functional validation.

Indexed as

CitrusGene Expression Regulation, PlantMultigene FamilyPlant ProteinsChromosomes, PlantDisease ResistanceGene Expression ProfilingGenome, PlantPhylogenyPlant DiseasesPlant ProteinsCitrus clementinaDisease resistant breedingHuanglongbingNBS-LRR gene family

Identifiers

PMID42129278
PMCPMC13357762

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