Stable Transfection of CD74 Gene Variants into A549 Lung Adenocarcinoma Cell Line.

Jasmine Vargas, Georgios Pantouris

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Jasmine VargasDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA.

Georgios PantourisDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA. gpantouris@pacific.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transfection is the introduction of foreign genetic material (e.g., DNA) into eukaryotic cells with the downstream goal of the expression and characterization of a protein with a specific biological function. This experimental technique is particularly useful in cancer research as it permits the study of oncogenes. When the foreign genetic material is incorporated into the genome of the host cell, then the technique is called "Stable Transfection." Once the transfected DNA has been integrated, the gene of interest is permanently expressed, allowing for research to be conducted over extended periods of time without disruption. Here, we describe the lipid-based stable transfection of CD74 gene variants into the A549 lung adenocarcinoma cell line. CD74 is a type II cell surface receptor with a particular interest in cancer biology due to its key functional role in both solid and hematological malignancies. Our protocol provides a highly reproducible, step-by-step procedure for stable transfection that can be applied to a variety of genes.

Indexed as

AdenocarcinomaAntigens, Differentiation, B-LymphocyteLung NeoplasmsTransfectionA549 CellsAdenocarcinoma of LungHistocompatibility Antigens Class IIHumansAntigens, Differentiation, B-LymphocyteHistocompatibility Antigens Class IIinvariant chainA549CancerCD74Cell-basedG418 selection antibioticGenomic integrationLipid-basedLung adenocarcinomaMammalian cell cultureStable transfection

Identifiers

PMID42129125

DOI10.1007/s00216-010-3821-6

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.