ArticleMethods in molecular biology (Clifton, N.J.)2026
Using Luciferase Reporter Cells to Assess the Effects of MIF Inhibitors on NF-κB Activation.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
Nuclear factor-κB (NF-κB) is a family of transcription factors that regulate cellular responses to a variety of stimuli, including stress, ultraviolet radiation, microbial components, growth factors, and cytokines. Assessment of NF-κB activation can be investigated by measuring the expression of NF-κB-responsive genes and their products, typically using polymerase chain reaction (PCR) and/or immunoblotting for protein. Alternatively, translocation of NF-κB subunits from the cytosol to the nucleus can be measured using microscopy techniques. However, these methods can be complex and time-consuming. Luciferase-based reporter systems allow for rapid detection of gene expression. This chapter details the use of myeloid cells stably transfected with NF-κB-inducible luciferase reporter constructs to assess the effects of inflammatory stimuli and MIF inhibitors on NF-κB activation.
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