Protocols for Assessing the Nuclease Activity of MIF.

Longfei Li, Seo-Hyun Kim, Ted M Dawson, Valina L Dawson

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Longfei LiInstitute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Seo-Hyun KimInstitute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Ted M DawsonInstitute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Valina L DawsonInstitute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. vdawson@jhmi.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Macrophage migration inhibitory factor (MIF) exhibits nuclease activity critical in DNA damage pathways linked to neurodegenerative diseases and cancer. Here, we describe dual approaches for measuring this nuclease activity, combining biochemical and cellular analysis. Recombinant MIF is subjected to enzymatic assays with L1 DNA substrates, analyzing cleavage patterns via agarose gel electrophoresis and time-dependent kinetics over 5-30 min. SH-SY5Y cells treated with the DNA-damaging agent MNNG, with or without the MIF nuclease inhibitor PAANIB-1, undergo pulse-field gel electrophoresis to assess DNA fragmentation following cellular stress. These assays can be used to test MIF nuclease activity and screen MIF nuclease activity inhibitors.

Indexed as

DeoxyribonucleasesEnzyme AssaysIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsCell Line, TumorDNADNA DamageDNA FragmentationElectrophoresis, Agar GelHumansDeoxyribonucleasesDNAIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMIF protein, humanDNA damageDNA fragmentationMIFPAANIB-1

Identifiers

PMID42129117

DOI10.1016/j.cell.2022.04.020

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.