ArticleMethods in molecular biology (Clifton, N.J.)2026
Assessing the Tautomerase Activity of MIF Using L-Dopachrome.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine implicated in the pathogenesis of multiple acute and chronic inflammatory conditions, harbours diverse catalytic activity, notably the ability to tautomerize 2-carboxy-2,3-dihydroxyindole-5,6-quinone (L-dopachrome) to its dihydroxyindole derivative. While an endogenous substrate for this tautomerase activity is yet to be fully characterized, it is possible MIF enzymatic activity is important for numerous biological functions. As a result, assessing the tautomerase activity of MIF may serve as a useful screening tool for identifying small-molecule MIF inhibitors. This chapter details the use of a simple, cell-free, spectrophotometric assay for investigating the inhibitory potential of small-molecule compounds against the keto-enol tautomerase activity of MIF.
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