Expression and Purification of Recombinant D-Dopachrome Tautomerase (D-DT).

Emmanuel K Yeboah, Samie Elmazi, Stephen J Headey, Natalie A Borg

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Emmanuel K YeboahImmunity and Immune Evasion Laboratory, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, 3083, Australia.

Samie ElmaziImmunity and Immune Evasion Laboratory, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, 3083, Australia.

Stephen J HeadeySchool of Science, RMIT University, Melbourne, VIC, 3000, Australia.

Natalie A BorgImmunity and Immune Evasion Laboratory, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, 3083, Australia. natalie.borg@rmit.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

D-dopachrome tautomerase (D-DT) is a multifunctional protein with various physiological roles, including immune system regulation and protection from oxidative stress. However, D-DT is also implicated in the progression of inflammatory conditions, cancers, and neurodegenerative diseases, where oxidative stress contributes to neuronal damage. Despite its importance, D-DT's role in cellular processes and disease remains to be fully elucidated. For both basic research and exploring D-DT as a potential therapeutic target, access to recombinant, purified D-DT is invaluable. We present a detailed methodology for the bacterial expression of D-DT and its purification using ion exchange and size exclusion chromatography. Our systematic approach yields 8 mg of purified protein per liter of bacterial culture.

Indexed as

Intramolecular OxidoreductasesRecombinant ProteinsChromatography, GelChromatography, Ion ExchangeEscherichia coliGene ExpressionHumansdopachrome isomeraseIntramolecular OxidoreductasesRecombinant ProteinsD-dopachrome tautomeraseD-DTPurificationRecombinant protein expression

Identifiers

PMID42129108

DOI10.1016/j.eclinm.2023.102375

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