Generation of MIF Peptides via Solid Phase Peptide Synthesis.

Aliyah Veronica R Pilien, Polina Eidelberg, Yadwinder S Mann, Georgios Pantouris, Jianhua Ren

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Aliyah Veronica R PilienDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA.

Polina EidelbergDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA.

Yadwinder S MannDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA.

Georgios PantourisDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA. gpantouris@pacific.edu.

Jianhua RenDepartment of Chemistry, University of the Pacific, Stockton, CA, 95211, USA. jren@pacific.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Solid phase peptide synthesis is a method used to generate peptides through the sequential addition of amino acids attached to an immobilized resin. Due to the procedure followed, excess reagents and byproducts are removed while synthesis is ongoing, resulting in a final product that is reproducibly obtained at high purity. Herein, we describe the synthetic procedure of peptides derived from the pleotropic protein macrophage migration inhibitory factor (MIF). Notably, this protocol is valid for the generation of peptides regardless of their sequence and length. Whereas MIF has diverse activities and protein partners, the generation of thoughtfully designed MIF peptides may have applications in drug discovery and structural biology.

Indexed as

Macrophage Migration-Inhibitory FactorsPeptidesSolid-Phase Synthesis TechniquesAmino Acid SequenceHumansMacrophage Migration-Inhibitory FactorsPeptidesImmobilized resinMacrophage migration inhibitory factorMass spectrometryPeptideSolid phase peptide synthesis

Identifiers

PMID42129107

DOI10.3389/fmolb.2022.783669

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.